Age-dependent effects of repeated immunization with a first generation adenovirus vector on the immune response and transgene expression in young and old rats

Irene Massari, Alessia Donnini, Katy Argentati, Stefania Straino, Antonella Mangoni, Carlo Gaetano, Claudio Viticchi, Maurizio C. Capogrossi, Mauro Provinciali

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We evaluated the kinetics of transgene expression and humoral and cellular immune responses against viral antigens and the product of the reporter gene LacZ in young (4 months) and old (20 months) Wistar rats. Animals received the intramuscular injection of a recombinant E1-deleted human type 5 adenovirus encoding β-gal (Ad-LacZ) on days 0 and 30. The transgene expression evaluated on day 2 after infection revealed a significantly higher β-gal activity in young than in old animals (1.9-fold increase, p

Original languageEnglish
Pages (from-to)823-831
Number of pages9
JournalExperimental Gerontology
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 1 2002

Fingerprint

Immunization
Transgenes
Adenoviridae
Rats
Animals
Human Adenoviruses
Viral Antigens
Intramuscular Injections
Humoral Immunity
Reporter Genes
Cellular Immunity
Wistar Rats
Genes
Kinetics
Infection

Keywords

  • Adenovirus vector
  • Aging
  • Gene therapy
  • Immune response

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Age-dependent effects of repeated immunization with a first generation adenovirus vector on the immune response and transgene expression in young and old rats. / Massari, Irene; Donnini, Alessia; Argentati, Katy; Straino, Stefania; Mangoni, Antonella; Gaetano, Carlo; Viticchi, Claudio; Capogrossi, Maurizio C.; Provinciali, Mauro.

In: Experimental Gerontology, Vol. 37, No. 6, 01.06.2002, p. 823-831.

Research output: Contribution to journalArticle

Massari, Irene ; Donnini, Alessia ; Argentati, Katy ; Straino, Stefania ; Mangoni, Antonella ; Gaetano, Carlo ; Viticchi, Claudio ; Capogrossi, Maurizio C. ; Provinciali, Mauro. / Age-dependent effects of repeated immunization with a first generation adenovirus vector on the immune response and transgene expression in young and old rats. In: Experimental Gerontology. 2002 ; Vol. 37, No. 6. pp. 823-831.
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