Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study

Fabio Ciccarone, Marco Malavolta, Roberta Calabrese, Tiziana Guastafierro, Maria Giulia Bacalini, Anna Reale, Claudio Franceschi, Miriam Capri, Antti Hervonen, Mikko Hurme, Beatrix Grubeck-Loebenstein, Bernhard Koller, Jürgen Bernhardt, Christiane Schӧn, Pieternella Eline Slagboom, Olivier Toussaint, Ewa Sikora, Efstathios S. Gonos, Nicolle Breusing, Tilman GruneEugène H J M Jansen, Martijn Dollé, Maria Moreno-Villanueva, Thilo Sindlinger, Alexander Bürkle, Michele Zampieri, Paola Caiafa

Research output: Contribution to journalArticle

Abstract

Aging is associated with alterations in the content and patterns of DNA methylation virtually throughout the entire human lifespan. Reasons for these variations are not well understood. However, several lines of evidence suggest that the epigenetic instability in aging may be traced back to the alteration of the expression of DNA methyltransferases. Here, the association of the expression of DNA methyltransferases DNMT1 and DNMT3B with age has been analysed in the context of the MARK-AGE study, a large-scale cross-sectional study of the European general population. Using peripheral blood mononuclear cells, we assessed the variation of DNMT1 and DNMT3B gene expression in more than two thousand age-stratified women and men (35–75 years) recruited across eight European countries. Significant age-related changes were detected for both transcripts. The level of DNMT1 gradually dropped with aging but this was only observed up to the age of 64 years. By contrast, the expression of DNMT3B decreased linearly with increasing age and this association was particularly evident in females. We next attempted to trace the age-related changes of both transcripts to the influence of different variables that have an impact on changes of their expression in the population, including demographics, dietary and health habits, and clinical parameters. Our results indicate that age affects the expression of DNMT1 and DNMT3B as an almost independent variable in respect of all other variables evaluated.

Original languageEnglish
Pages (from-to)755-765
Number of pages11
JournalAging Cell
Volume15
Issue number4
DOIs
Publication statusPublished - Aug 1 2016

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Methyltransferases
DNA
Feeding Behavior
DNA Methylation
Epigenomics
Population
Blood Cells
Cross-Sectional Studies
Demography
Gene Expression
Health

Keywords

  • aging
  • DNA methylation
  • DNMT1
  • DNMT3B

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

Cite this

Ciccarone, F., Malavolta, M., Calabrese, R., Guastafierro, T., Bacalini, M. G., Reale, A., ... Caiafa, P. (2016). Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study. Aging Cell, 15(4), 755-765. https://doi.org/10.1111/acel.12485

Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study. / Ciccarone, Fabio; Malavolta, Marco; Calabrese, Roberta; Guastafierro, Tiziana; Bacalini, Maria Giulia; Reale, Anna; Franceschi, Claudio; Capri, Miriam; Hervonen, Antti; Hurme, Mikko; Grubeck-Loebenstein, Beatrix; Koller, Bernhard; Bernhardt, Jürgen; Schӧn, Christiane; Slagboom, Pieternella Eline; Toussaint, Olivier; Sikora, Ewa; Gonos, Efstathios S.; Breusing, Nicolle; Grune, Tilman; Jansen, Eugène H J M; Dollé, Martijn; Moreno-Villanueva, Maria; Sindlinger, Thilo; Bürkle, Alexander; Zampieri, Michele; Caiafa, Paola.

In: Aging Cell, Vol. 15, No. 4, 01.08.2016, p. 755-765.

Research output: Contribution to journalArticle

Ciccarone, F, Malavolta, M, Calabrese, R, Guastafierro, T, Bacalini, MG, Reale, A, Franceschi, C, Capri, M, Hervonen, A, Hurme, M, Grubeck-Loebenstein, B, Koller, B, Bernhardt, J, Schӧn, C, Slagboom, PE, Toussaint, O, Sikora, E, Gonos, ES, Breusing, N, Grune, T, Jansen, EHJM, Dollé, M, Moreno-Villanueva, M, Sindlinger, T, Bürkle, A, Zampieri, M & Caiafa, P 2016, 'Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study', Aging Cell, vol. 15, no. 4, pp. 755-765. https://doi.org/10.1111/acel.12485
Ciccarone, Fabio ; Malavolta, Marco ; Calabrese, Roberta ; Guastafierro, Tiziana ; Bacalini, Maria Giulia ; Reale, Anna ; Franceschi, Claudio ; Capri, Miriam ; Hervonen, Antti ; Hurme, Mikko ; Grubeck-Loebenstein, Beatrix ; Koller, Bernhard ; Bernhardt, Jürgen ; Schӧn, Christiane ; Slagboom, Pieternella Eline ; Toussaint, Olivier ; Sikora, Ewa ; Gonos, Efstathios S. ; Breusing, Nicolle ; Grune, Tilman ; Jansen, Eugène H J M ; Dollé, Martijn ; Moreno-Villanueva, Maria ; Sindlinger, Thilo ; Bürkle, Alexander ; Zampieri, Michele ; Caiafa, Paola. / Age-dependent expression of DNMT1 and DNMT3B in PBMCs from a large European population enrolled in the MARK-AGE study. In: Aging Cell. 2016 ; Vol. 15, No. 4. pp. 755-765.
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AU - Grune, Tilman

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