Age-dependent increases in oxidative damage to DNA, lipids, and proteins in human skeletal muscle

Patrizia Mecocci, Giorgio Fanó, Stefania Fulle, Usha MacGarvey, Leslie Shinobu, M. Cristina Polidori, Antonio Cherubini, Jacopo Vecchiet, Umberto Senin, M. Flint Beal

Research output: Contribution to journalArticle

Abstract

A role for oxidative damage in normal aging is supported by studies in experimental animals, but there is limited evidence in man. We examined markers of oxidative damage to DNA, lipids, and proteins in 66 muscle biopsy specimens from humans aged 25 to 93 years. There were age-dependent increases in 8-hydroxy-2-deoxyguanosine (OH8dG), a marker of oxidative damage to DNA, in malondialdehyde (MDA), a marker of lipid peroxidation, and to a lesser extent in protein carbonyl groups, a marker of protein oxidation. The increases in OH8dG were significantly correlated with increases in MDA. These results provide evidence for a role of oxidative damage in human aging which may contribute to age-dependent losses of muscle strength and stamina.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalFree Radical Biology and Medicine
Volume26
Issue number3-4
DOIs
Publication statusPublished - Feb 1999

Keywords

  • 8-Hydroxy-2- deoxyguanosine
  • Aging
  • Free radicals
  • Malondialdehyde
  • Muscle oxidative damage
  • Protein carbonyls

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

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    Mecocci, P., Fanó, G., Fulle, S., MacGarvey, U., Shinobu, L., Polidori, M. C., Cherubini, A., Vecchiet, J., Senin, U., & Beal, M. F. (1999). Age-dependent increases in oxidative damage to DNA, lipids, and proteins in human skeletal muscle. Free Radical Biology and Medicine, 26(3-4), 303-308. https://doi.org/10.1016/S0891-5849(98)00208-1