Age related changes of the mitochondrial energy metabolism in rat liver and heart

G. Capozza, F. Guerrieri, G. Vendemiale, E. Altomare, S. Papa

Research output: Contribution to journalArticle

Abstract

The influence of aging on mitochondrial energy metabolism of rat liver and rat heart has been studied by analysis of (i) respiratory rate of succinate-supplemented mitochondria in state III (coupled state in the presence of ADP + Pi); (ii) the rate of synthesis of ATP in succinate-supplemented mitochondria; (iii) the ATP hydrolase activity of sonicated submitochondrial particles. The results indicate a decrease of the F0F1-ATP synthase activity in mitochondria isolated from both organs of aged (24-month-old) as compared to young (3-month-old) rats which was accompanied by a decrease of immunodetected amount of the β-F1 (subunit of the catalytic F1 sector of F0F1-ATP synthase). These effects were more evident in heart than in liver mitochondria. Analysis of the mitochondrial content of glutathione (GSH), an essential intracellular antioxidant agent, shows a decrease in mitochondria of both tissues of aged animals. Exposure of submitochondrial particles to free radicals, produced either by 60Co or by respirtory chain (in presence of the inhibitor antimycin A) mimicked the alterations of F0F1 ATP synthase obsreved in submitochondrial particles of aged rats. The possible relationship between aging process, free radical production and alteration of mitochondrial oxidative phosphorylation is discussed.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalArchives of Gerontology and Geriatrics
Volume19
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 1994

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Energy Metabolism
Submitochondrial Particles
Adenosine Triphosphate
energy
Mitochondria
Liver
Succinic Acid
animal
Free Radicals
Antimycin A
Liver Mitochondrion
Oxidative Phosphorylation
Hydrolases
Respiratory Rate
Adenosine Diphosphate
Glutathione
Catalytic Domain
Antioxidants

Keywords

  • ATP synthase
  • free radicals
  • glutathione
  • oxidative phosphorylation

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology
  • Gerontology
  • Health(social science)
  • Medicine(all)

Cite this

Age related changes of the mitochondrial energy metabolism in rat liver and heart. / Capozza, G.; Guerrieri, F.; Vendemiale, G.; Altomare, E.; Papa, S.

In: Archives of Gerontology and Geriatrics, Vol. 19, No. SUPPL. 1, 1994, p. 31-38.

Research output: Contribution to journalArticle

Capozza, G. ; Guerrieri, F. ; Vendemiale, G. ; Altomare, E. ; Papa, S. / Age related changes of the mitochondrial energy metabolism in rat liver and heart. In: Archives of Gerontology and Geriatrics. 1994 ; Vol. 19, No. SUPPL. 1. pp. 31-38.
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