Age-related differences in the expression of circulating microRNAs: miR-21 as a new circulating marker of inflammaging

Fabiola Olivieri, Liana Spazzafumo, Gabriele Santini, Raffaella Lazzarini, Maria Cristina Albertini, Maria Rita Rippo, Roberta Galeazzi, Angela Marie Abbatecola, Fiorella Marcheselli, Daniela Monti, Rita Ostan, Elisa Cevenini, Roberto Antonicelli, Claudio Franceschi, Antonio Domenico Procopio

Research output: Contribution to journalArticle

Abstract

Circulating microRNAs (miRs) have been investigated as diagnostic/prognostic biomarkers in human diseases. However, little is known about their expression throughout the aging process.Eleven healthy individuals aged 20, 80 and 100. years underwent miR plasma profiling. The validation cohort consisted of 111 healthy adults (CTR) aged 20-105. years and included 30 centenarians. In addition, 34 patients with cardiovascular disease (CVD) and 15 healthy centenarian offspring (CO) were enrolled.An exploratory factorial analysis grouped the miRs into three main factors: factor 1 primarily higher in 20-year-old subjects, but these differences did not reach statistical significance, factor 2 primarily higher in octogenarians and factor 3 primarily higher in centenarians. MiR-21, the most highly expressed miR of factors 2 and 3, was further validated, confirming the differences in the age groups. MiR-21 expression was higher in the CVD patients and lower in the CO compared to the age-matched CTR. MiR-21 was correlated with C-reactive protein and fibrinogen levels. TGF-β signaling was the predicted common pathway targeted by miRs of factors 2 and 3. TGF-βR2 mRNA, a validated miR-21 target, showed the highest expression in the leukocytes from a subset of the octogenarians.Our findings suggest that miR-21 may be a new biomarker of inflammation.

Original languageEnglish
Pages (from-to)675-685
Number of pages11
JournalMechanisms of Ageing and Development
Volume133
Issue number11-12
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Centenarians
  • Centenarians offspring
  • Circulating microRNA
  • Circulating miR-21

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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