TY - JOUR
T1 - Age-related differences in the expression of circulating microRNAs
T2 - miR-21 as a new circulating marker of inflammaging
AU - Olivieri, Fabiola
AU - Spazzafumo, Liana
AU - Santini, Gabriele
AU - Lazzarini, Raffaella
AU - Albertini, Maria Cristina
AU - Rippo, Maria Rita
AU - Galeazzi, Roberta
AU - Abbatecola, Angela Marie
AU - Marcheselli, Fiorella
AU - Monti, Daniela
AU - Ostan, Rita
AU - Cevenini, Elisa
AU - Antonicelli, Roberto
AU - Franceschi, Claudio
AU - Procopio, Antonio Domenico
PY - 2012/11
Y1 - 2012/11
N2 - Circulating microRNAs (miRs) have been investigated as diagnostic/prognostic biomarkers in human diseases. However, little is known about their expression throughout the aging process.Eleven healthy individuals aged 20, 80 and 100. years underwent miR plasma profiling. The validation cohort consisted of 111 healthy adults (CTR) aged 20-105. years and included 30 centenarians. In addition, 34 patients with cardiovascular disease (CVD) and 15 healthy centenarian offspring (CO) were enrolled.An exploratory factorial analysis grouped the miRs into three main factors: factor 1 primarily higher in 20-year-old subjects, but these differences did not reach statistical significance, factor 2 primarily higher in octogenarians and factor 3 primarily higher in centenarians. MiR-21, the most highly expressed miR of factors 2 and 3, was further validated, confirming the differences in the age groups. MiR-21 expression was higher in the CVD patients and lower in the CO compared to the age-matched CTR. MiR-21 was correlated with C-reactive protein and fibrinogen levels. TGF-β signaling was the predicted common pathway targeted by miRs of factors 2 and 3. TGF-βR2 mRNA, a validated miR-21 target, showed the highest expression in the leukocytes from a subset of the octogenarians.Our findings suggest that miR-21 may be a new biomarker of inflammation.
AB - Circulating microRNAs (miRs) have been investigated as diagnostic/prognostic biomarkers in human diseases. However, little is known about their expression throughout the aging process.Eleven healthy individuals aged 20, 80 and 100. years underwent miR plasma profiling. The validation cohort consisted of 111 healthy adults (CTR) aged 20-105. years and included 30 centenarians. In addition, 34 patients with cardiovascular disease (CVD) and 15 healthy centenarian offspring (CO) were enrolled.An exploratory factorial analysis grouped the miRs into three main factors: factor 1 primarily higher in 20-year-old subjects, but these differences did not reach statistical significance, factor 2 primarily higher in octogenarians and factor 3 primarily higher in centenarians. MiR-21, the most highly expressed miR of factors 2 and 3, was further validated, confirming the differences in the age groups. MiR-21 expression was higher in the CVD patients and lower in the CO compared to the age-matched CTR. MiR-21 was correlated with C-reactive protein and fibrinogen levels. TGF-β signaling was the predicted common pathway targeted by miRs of factors 2 and 3. TGF-βR2 mRNA, a validated miR-21 target, showed the highest expression in the leukocytes from a subset of the octogenarians.Our findings suggest that miR-21 may be a new biomarker of inflammation.
KW - Centenarians
KW - Centenarians offspring
KW - Circulating microRNA
KW - Circulating miR-21
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U2 - 10.1016/j.mad.2012.09.004
DO - 10.1016/j.mad.2012.09.004
M3 - Article
C2 - 23041385
AN - SCOPUS:84870741421
VL - 133
SP - 675
EP - 685
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 11-12
ER -