Age-related increases of 8-hydroxy-2'-deoxyguanosine and DNA-protein crosslinks in mouse organs

Alberto Izzotti, Cristina Cartiglia, Maurizio Taningher, Silvio De Flora, Roumen Balansky

Research output: Contribution to journalArticlepeer-review

Abstract

Experimental data suggest a possible role of DNA damage in aging, mainly related to oxidative lesions. With the objective of evaluating DNA lesions as molecular biomarkers of aging, we measured 8-hydroxy-2'-deoxyguanosine (8-OH-dG) and DNA-protein crosslinks (DPXL) levels in different organs of mice aged 12 and 24 months. 8-OH-dG was detected by 32P postlabelling after removing unmodified dG by trifluoracetic acid, which prevented the artificial formation of 8-OH-dG during 32P labelling procedures. Appreciable 8-OH-dG amounts were detected in 12-month-old mice in liver (1.8±0.7 8-OH-dG/105 normal nucleotides), brain (1.6±0.5) and heart (2.3±0.5). In 24-month-old mice these values were higher in all examined organs (liver, 2.7±0.4; brain, 3.6±1.1; heart, 6.8±2.2 8-OH-dG/105 normal nucleotides). This accounted for a 1.5-fold increase in liver (not significant), 2.3-fold increase in brain (P

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume446
Issue number2
DOIs
Publication statusPublished - Dec 13 1999

Keywords

  • 8-Hydroxy-2'-deoxyguanosine
  • P postlabelling
  • Aging
  • DNA-protein crosslinks
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Fingerprint Dive into the research topics of 'Age-related increases of 8-hydroxy-2'-deoxyguanosine and DNA-protein crosslinks in mouse organs'. Together they form a unique fingerprint.

Cite this