Age-related increases of 8-hydroxy-2'-deoxyguanosine and DNA-protein crosslinks in mouse organs

Alberto Izzotti, Cristina Cartiglia, Maurizio Taningher, Silvio De Flora, Roumen Balansky

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Experimental data suggest a possible role of DNA damage in aging, mainly related to oxidative lesions. With the objective of evaluating DNA lesions as molecular biomarkers of aging, we measured 8-hydroxy-2'-deoxyguanosine (8-OH-dG) and DNA-protein crosslinks (DPXL) levels in different organs of mice aged 12 and 24 months. 8-OH-dG was detected by 32P postlabelling after removing unmodified dG by trifluoracetic acid, which prevented the artificial formation of 8-OH-dG during 32P labelling procedures. Appreciable 8-OH-dG amounts were detected in 12-month-old mice in liver (1.8±0.7 8-OH-dG/105 normal nucleotides), brain (1.6±0.5) and heart (2.3±0.5). In 24-month-old mice these values were higher in all examined organs (liver, 2.7±0.4; brain, 3.6±1.1; heart, 6.8±2.2 8-OH-dG/105 normal nucleotides). This accounted for a 1.5-fold increase in liver (not significant), 2.3-fold increase in brain (P

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume446
Issue number2
DOIs
Publication statusPublished - Dec 13 1999

Fingerprint

DNA
Proteins
Liver
Brain
Nucleotides
8-oxo-7-hydrodeoxyguanosine
DNA Damage
hydroxide ion
Biomarkers
Acids

Keywords

  • 8-Hydroxy-2'-deoxyguanosine
  • P postlabelling
  • Aging
  • DNA-protein crosslinks
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

Age-related increases of 8-hydroxy-2'-deoxyguanosine and DNA-protein crosslinks in mouse organs. / Izzotti, Alberto; Cartiglia, Cristina; Taningher, Maurizio; De Flora, Silvio; Balansky, Roumen.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 446, No. 2, 13.12.1999, p. 215-223.

Research output: Contribution to journalArticle

Izzotti, Alberto ; Cartiglia, Cristina ; Taningher, Maurizio ; De Flora, Silvio ; Balansky, Roumen. / Age-related increases of 8-hydroxy-2'-deoxyguanosine and DNA-protein crosslinks in mouse organs. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 1999 ; Vol. 446, No. 2. pp. 215-223.
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