Abstract
Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.
| Original language | English |
|---|---|
| Pages (from-to) | 1268-1280 |
| Number of pages | 13 |
| Journal | Aging |
| Volume | 10 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 1 2018 |
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Keywords
- Age-related changes of M1/M2 circulating monocytes
ASJC Scopus subject areas
- Ageing
- Cell Biology
Cite this
Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals. / Costantini, Andrea; Viola, Nadia; Berretta, Antonella; Galeazzi, Roberta; Matacchione, Giulia; Sabbatinelli, Jacopo; Storci, Gianluca; De Matteis, Serena; Butini, Luca; Rippo, Maria Rita; Procopio, Antonio Domenico; Caraceni, Daniele; Antonicelli, Roberto; Olivieri, Fabiola; Bonafè, Massimiliano.
In: Aging, Vol. 10, No. 6, 01.06.2018, p. 1268-1280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals
AU - Costantini, Andrea
AU - Viola, Nadia
AU - Berretta, Antonella
AU - Galeazzi, Roberta
AU - Matacchione, Giulia
AU - Sabbatinelli, Jacopo
AU - Storci, Gianluca
AU - De Matteis, Serena
AU - Butini, Luca
AU - Rippo, Maria Rita
AU - Procopio, Antonio Domenico
AU - Caraceni, Daniele
AU - Antonicelli, Roberto
AU - Olivieri, Fabiola
AU - Bonafè, Massimiliano
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.
AB - Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.
KW - Age-related changes of M1/M2 circulating monocytes
UR - http://www.scopus.com/inward/record.url?scp=85049530055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049530055&partnerID=8YFLogxK
U2 - 10.18632/aging.101465
DO - 10.18632/aging.101465
M3 - Article
AN - SCOPUS:85049530055
VL - 10
SP - 1268
EP - 1280
JO - Aging
JF - Aging
SN - 1945-4589
IS - 6
ER -