Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains

Cristina Mazzaccara, Giuseppe Labruna, Gennaro Cito, Marzia Scarfò, Mario De Felice, Lucio Pastore, Lucia Sacchetti

Research output: Contribution to journalArticle

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Abstract

Background: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. Methodology/Principal Findings: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/ heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female) of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5th and 97.5th percentiles). The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001). Inter-strain differences were observed for: (1) GLU, t-Bil, K+, Ca++, PO4 - (p++ (p++, Na++, K+, Cl- (p

Original languageEnglish
Article numbere3772
JournalPLoS One
Volume3
Issue number11
DOIs
Publication statusPublished - Nov 20 2008

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Inbred C3H Mouse
Reference Values
normal values
Water-Electrolyte Balance
Blood Urea Nitrogen
mice
Animals
Blood
Quality Control
Lipoproteins
Pancreas
Myocardium
Animal Models
strain differences
blood
sinuses
Kidney
human diseases
Metabolism
pancreas

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains. / Mazzaccara, Cristina; Labruna, Giuseppe; Cito, Gennaro; Scarfò, Marzia; De Felice, Mario; Pastore, Lucio; Sacchetti, Lucia.

In: PLoS One, Vol. 3, No. 11, e3772, 20.11.2008.

Research output: Contribution to journalArticle

Mazzaccara, Cristina ; Labruna, Giuseppe ; Cito, Gennaro ; Scarfò, Marzia ; De Felice, Mario ; Pastore, Lucio ; Sacchetti, Lucia. / Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains. In: PLoS One. 2008 ; Vol. 3, No. 11.
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