Abstract
Background: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. Methodology/Principal Findings: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/ heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female) of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5th and 97.5th percentiles). The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001). Inter-strain differences were observed for: (1) GLU, t-Bil, K+, Ca++, PO4 - (p++ (p++, Na++, K+, Cl- (p
| Original language | English |
|---|---|
| Article number | e3772 |
| Journal | PLoS One |
| Volume | 3 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 20 2008 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains. / Mazzaccara, Cristina; Labruna, Giuseppe; Cito, Gennaro; Scarfò, Marzia; De Felice, Mario; Pastore, Lucio; Sacchetti, Lucia.
In: PLoS One, Vol. 3, No. 11, e3772, 20.11.2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains
AU - Mazzaccara, Cristina
AU - Labruna, Giuseppe
AU - Cito, Gennaro
AU - Scarfò, Marzia
AU - De Felice, Mario
AU - Pastore, Lucio
AU - Sacchetti, Lucia
PY - 2008/11/20
Y1 - 2008/11/20
N2 - Background: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. Methodology/Principal Findings: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/ heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female) of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5th and 97.5th percentiles). The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001). Inter-strain differences were observed for: (1) GLU, t-Bil, K+, Ca++, PO4 - (p++ (p++, Na++, K+, Cl- (p
AB - Background: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. Methodology/Principal Findings: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/ heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female) of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5th and 97.5th percentiles). The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001). Inter-strain differences were observed for: (1) GLU, t-Bil, K+, Ca++, PO4 - (p++ (p++, Na++, K+, Cl- (p
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U2 - 10.1371/journal.pone.0003772
DO - 10.1371/journal.pone.0003772
M3 - Article
VL - 3
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 11
M1 - e3772
ER -