Aging and airway inflammation

Giovanna Elisiana Carpagnano, Viviana Turchiarelli, Antonio Spanevello, Grazia Pia Palladino, Maria Pia Foschino Barbaro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Since little is known of airways inflammation in the elderly, we have carried out a study to explore the presence of some inflammatory markers in the airways of healthy subjects of different ages using a non-invasive method which is particularly suitable for aged people. Objective: The aim of this work was to investigate whether parameters, including (1) pH, IL-8 and TNF-α in exhaled breath condensate (EBC), (2) exhaled nitric oxide levels (NO), and (3) inflammatory cell profile in induced sputum, are age-related. Materials and methods: Thirty healthy adults (10 subjects below the age of 30 [A], 10 subjects between 30 and 60 years [B], and 10 subjects over 60 years of age [C]), were enrolled in the study. IL-8 and TNF-α levels were measured in breath condensate. Exhaled pH was measured after deaeration/decarbonation by means of a pH-meter. A rapid-response chemiluminescence NO analyzer was used to quantify NO. Induced sputum was collected, homogenized with dithiothreitol, and cytospins for differential cell were produced. Results: The levels of IL-8 and TNF-α in EBC, the levels of exhaled NO, and the percentage of neutrophils in induced sputum were significantly elevated in C and B compared with A; the EBC pH level was significantly reduced in C and B compared with A. The EBC levels of IL-8, TNF-α, pH, the level of exhaled NO, and the percentage of neutrophils correlated significantly with age. Conclusion: This study has shown the presence of age-related airways inflammation in healthy subjects.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalAging clinical and experimental research
Volume25
Issue number3
DOIs
Publication statusPublished - 2013

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Nitric Oxide
Interleukin-8
Inflammation
Sputum
Healthy Volunteers
Neutrophils
Dithiothreitol
Luminescence

Keywords

  • Airways inflammation
  • Elderly
  • Exhaled breath condensate
  • Exhaled nitric oxide
  • Induced sputum

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology
  • Medicine(all)

Cite this

Carpagnano, G. E., Turchiarelli, V., Spanevello, A., Palladino, G. P., & Barbaro, M. P. F. (2013). Aging and airway inflammation. Aging clinical and experimental research, 25(3), 239-245. https://doi.org/10.1007/s40520-013-0040-y

Aging and airway inflammation. / Carpagnano, Giovanna Elisiana; Turchiarelli, Viviana; Spanevello, Antonio; Palladino, Grazia Pia; Barbaro, Maria Pia Foschino.

In: Aging clinical and experimental research, Vol. 25, No. 3, 2013, p. 239-245.

Research output: Contribution to journalArticle

Carpagnano, GE, Turchiarelli, V, Spanevello, A, Palladino, GP & Barbaro, MPF 2013, 'Aging and airway inflammation', Aging clinical and experimental research, vol. 25, no. 3, pp. 239-245. https://doi.org/10.1007/s40520-013-0040-y
Carpagnano, Giovanna Elisiana ; Turchiarelli, Viviana ; Spanevello, Antonio ; Palladino, Grazia Pia ; Barbaro, Maria Pia Foschino. / Aging and airway inflammation. In: Aging clinical and experimental research. 2013 ; Vol. 25, No. 3. pp. 239-245.
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