Aging and corneal layers

an in vivo corneal confocal microscopy study

Catia Gambato, Evelyn Longhin, Anton Giulio Catania, Daniela Lazzarini, Raffaele Parrozzani, Edoardo Midena

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: To describe age-related changes of different corneal layers using a quantitative analysis of in vivo corneal confocal microscopy.

Design: Descriptive observational cross-sectional study.

Methods: A total of 108 healthy corneas of 108 subjects, distributed in four age categories, underwent in vivo corneal confocal microscopy. The effect of aging on the main features of corneal epithelium, sub-basal nerve plexus, stroma, and endothelium was investigated.

Results: Mean diameter of superficial epithelial cells increases with age (0.05 μm per year; p <0.0001). Mean cell density of basal epithelium does not change with age (p = 0.37). The sub-basal nerve plexus fiber number, density, and the number of beadings do not statistically change with age (p = 0.14, p = 0.10 and p = 0.17, respectively). Keratocyte density significantly reduces with age in each stromal layer (p <0.0001). Endothelial cell count decreases by 10.92 cells/mm2 per year (p <0.0001). Endothelial polymegathism index and pleomorphism index do not change with age (p = 0.79 and p = 0.39, respectively).

Conclusions: Corneal confocal microscopy allows a non-invasive examination of the living cornea, analyzing the microstructure of each corneal layer. Aging significantly influences the corneal confocal microscopy parameters of individual corneal layers, except sub-basal nerve plexus and basal epithelium.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume253
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Confocal Microscopy
Cornea
Epithelium
Cell Count
Corneal Epithelium
Nerve Fibers
Endothelium
Endothelial Cells
Cross-Sectional Studies
Epithelial Cells

Keywords

  • Confocal microscopy
  • Corneal aging
  • Healthy cornea

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Aging and corneal layers : an in vivo corneal confocal microscopy study. / Gambato, Catia; Longhin, Evelyn; Catania, Anton Giulio; Lazzarini, Daniela; Parrozzani, Raffaele; Midena, Edoardo.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 253, No. 2, 2014, p. 267-275.

Research output: Contribution to journalArticle

Gambato, Catia ; Longhin, Evelyn ; Catania, Anton Giulio ; Lazzarini, Daniela ; Parrozzani, Raffaele ; Midena, Edoardo. / Aging and corneal layers : an in vivo corneal confocal microscopy study. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2014 ; Vol. 253, No. 2. pp. 267-275.
@article{a8a4f2a6aafb48ce8abe2010c3a52ab2,
title = "Aging and corneal layers: an in vivo corneal confocal microscopy study",
abstract = "Purpose: To describe age-related changes of different corneal layers using a quantitative analysis of in vivo corneal confocal microscopy.Design: Descriptive observational cross-sectional study.Methods: A total of 108 healthy corneas of 108 subjects, distributed in four age categories, underwent in vivo corneal confocal microscopy. The effect of aging on the main features of corneal epithelium, sub-basal nerve plexus, stroma, and endothelium was investigated.Results: Mean diameter of superficial epithelial cells increases with age (0.05 μm per year; p <0.0001). Mean cell density of basal epithelium does not change with age (p = 0.37). The sub-basal nerve plexus fiber number, density, and the number of beadings do not statistically change with age (p = 0.14, p = 0.10 and p = 0.17, respectively). Keratocyte density significantly reduces with age in each stromal layer (p <0.0001). Endothelial cell count decreases by 10.92 cells/mm2 per year (p <0.0001). Endothelial polymegathism index and pleomorphism index do not change with age (p = 0.79 and p = 0.39, respectively).Conclusions: Corneal confocal microscopy allows a non-invasive examination of the living cornea, analyzing the microstructure of each corneal layer. Aging significantly influences the corneal confocal microscopy parameters of individual corneal layers, except sub-basal nerve plexus and basal epithelium.",
keywords = "Confocal microscopy, Corneal aging, Healthy cornea",
author = "Catia Gambato and Evelyn Longhin and Catania, {Anton Giulio} and Daniela Lazzarini and Raffaele Parrozzani and Edoardo Midena",
year = "2014",
doi = "10.1007/s00417-014-2812-2",
language = "English",
volume = "253",
pages = "267--275",
journal = "Graefe's Archive for Clinical and Experimental Ophthalmology",
issn = "0721-832X",
number = "2",

}

TY - JOUR

T1 - Aging and corneal layers

T2 - an in vivo corneal confocal microscopy study

AU - Gambato, Catia

AU - Longhin, Evelyn

AU - Catania, Anton Giulio

AU - Lazzarini, Daniela

AU - Parrozzani, Raffaele

AU - Midena, Edoardo

PY - 2014

Y1 - 2014

N2 - Purpose: To describe age-related changes of different corneal layers using a quantitative analysis of in vivo corneal confocal microscopy.Design: Descriptive observational cross-sectional study.Methods: A total of 108 healthy corneas of 108 subjects, distributed in four age categories, underwent in vivo corneal confocal microscopy. The effect of aging on the main features of corneal epithelium, sub-basal nerve plexus, stroma, and endothelium was investigated.Results: Mean diameter of superficial epithelial cells increases with age (0.05 μm per year; p <0.0001). Mean cell density of basal epithelium does not change with age (p = 0.37). The sub-basal nerve plexus fiber number, density, and the number of beadings do not statistically change with age (p = 0.14, p = 0.10 and p = 0.17, respectively). Keratocyte density significantly reduces with age in each stromal layer (p <0.0001). Endothelial cell count decreases by 10.92 cells/mm2 per year (p <0.0001). Endothelial polymegathism index and pleomorphism index do not change with age (p = 0.79 and p = 0.39, respectively).Conclusions: Corneal confocal microscopy allows a non-invasive examination of the living cornea, analyzing the microstructure of each corneal layer. Aging significantly influences the corneal confocal microscopy parameters of individual corneal layers, except sub-basal nerve plexus and basal epithelium.

AB - Purpose: To describe age-related changes of different corneal layers using a quantitative analysis of in vivo corneal confocal microscopy.Design: Descriptive observational cross-sectional study.Methods: A total of 108 healthy corneas of 108 subjects, distributed in four age categories, underwent in vivo corneal confocal microscopy. The effect of aging on the main features of corneal epithelium, sub-basal nerve plexus, stroma, and endothelium was investigated.Results: Mean diameter of superficial epithelial cells increases with age (0.05 μm per year; p <0.0001). Mean cell density of basal epithelium does not change with age (p = 0.37). The sub-basal nerve plexus fiber number, density, and the number of beadings do not statistically change with age (p = 0.14, p = 0.10 and p = 0.17, respectively). Keratocyte density significantly reduces with age in each stromal layer (p <0.0001). Endothelial cell count decreases by 10.92 cells/mm2 per year (p <0.0001). Endothelial polymegathism index and pleomorphism index do not change with age (p = 0.79 and p = 0.39, respectively).Conclusions: Corneal confocal microscopy allows a non-invasive examination of the living cornea, analyzing the microstructure of each corneal layer. Aging significantly influences the corneal confocal microscopy parameters of individual corneal layers, except sub-basal nerve plexus and basal epithelium.

KW - Confocal microscopy

KW - Corneal aging

KW - Healthy cornea

UR - http://www.scopus.com/inward/record.url?scp=84925537845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925537845&partnerID=8YFLogxK

U2 - 10.1007/s00417-014-2812-2

DO - 10.1007/s00417-014-2812-2

M3 - Article

VL - 253

SP - 267

EP - 275

JO - Graefe's Archive for Clinical and Experimental Ophthalmology

JF - Graefe's Archive for Clinical and Experimental Ophthalmology

SN - 0721-832X

IS - 2

ER -