Airborne particulate matter and mitochondrial damage: A cross-sectional study

Lifang Hou, Zhong Zheng Zhu, Xiao Zhang, Francesco Nordio, Matteo Bonzini, Joel Schwartz, Mirjam Hoxha, Laura Dioni, Barbara Marinelli, Valeria Pegoraro, Pietro Apostoli, Pier Alberto Bertazzi, Andrea Baccarelli

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Oxidative stress generation is a primary mechanism mediating the effects of Particulate Matter (PM) on human health. Although mitochondria are both the major intracellular source and target of oxidative stress, the effect of PM on mitochondria has never been evaluated in exposed individuals. Methods. In 63 male healthy steel workers from Brescia, Italy, studied between April and May 2006, we evaluated whether exposure to PM was associated with increased mitochondrial DNA copy number (MtDNAcn), an established marker of mitochondria damage and malfunctioning. Relative MtDNAcn (RMtDNAcn) was determined by real-time PCR in blood DNA obtained on the 1st (time 1) and 4th day (time 2) of the same work week. Individual exposures to PM10, PM1, coarse particles (PM10-PM 1) and airborne metal components of PM10 (chromium, lead, arsenic, nickel, manganese) were estimated based on measurements in the 11 work areas and time spent by the study subjects in each area. Results. RMtDNAcn was higher on the 4th day (mean = 1.31; 95%CI = 1.22 to 1.40) than on the 1st day of the work week (mean = 1.09; 95%CI = 1.00 to 1.17). PM exposure was positively associated with RMtDNAcn on either the 4th (PM10: = 0.06, 95%CI = -0.06 to 0.17; PM1: = 0.08, 95%CI = -0.08 to 0.23; coarse: = 0.06, 95%CI = -0.06 to 0.17) or the 1st day (PM10: = 0.18, 95%CI = 0.09 to 0.26; PM1: = 0.23, 95%CI = 0.11 to 0.35; coarse: = 0.17, 95%CI = 0.09 to 0.26). Metal concentrations were not associated with RMtDNAcn. Conclusions. PM exposure is associated with damaged mitochondria, as reflected in increased MtDNAcn. Damaged mitochondria may intensify oxidative-stress production and effects.

Original languageEnglish
Article number48
JournalEnvironmental Health: A Global Access Science Source
Volume9
Issue number1
DOIs
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Medicine(all)

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