AIRE polymorphism, melanoma antigen-specific T cell immunity, and susceptibility to melanoma

Giuseppina Conteduca, Daniela Fenoglio, Alessia Parodi, Florinda Battaglia, Francesca Kalli, Simone Negrini, Samuele Tardito, Francesca Ferrera, Annalisa Salis, Enrico Millo, Giuseppe Pasquale, Giusi Barra, Gianluca Damonte, Francesco Indiveri, Soldano Ferrone, Gilberto Filaci

Research output: Contribution to journalArticlepeer-review

Abstract

AIRE is involved in susceptibility to melanoma perhaps regulating T cell immunity against melanoma antigens (MA). To address this issue, AIRE and MAGEB2 expressions were measured by real time PCR in medullary thymic epithelial cells (mTECs) from two strains of C57BL/6 mice bearing either T or C allelic variant of the rs1800522 AIRE SNP. Moreover, the extent of apoptosis induced by mTECs in MAGEB2-specific T cells and the susceptibility to in vivo melanoma B16F10 cell challenge were compared in the two mouse strains. The C allelic variant, protective in humans against melanoma, induced lower AIRE and MAGEB2 expression in C57BL/6 mouse mTECs than the T allele. Moreover, mTECs expressing the C allelic variant induced lower extent of apoptosis in MAGEB2-specific syngeneic T cells than mTECs bearing the T allelic variant (p < 0.05). Vaccination against MAGEB2 induced higher frequency of MAGEB2-specific CTL and exerted higher protective effect against melanoma development in mice bearing the CC AIRE genotype than in those bearing the TT one (p < 0.05). These findings show that allelic variants of one AIRE SNP may differentially shape the MA-specific T cell repertoire potentially influencing susceptibility to melanoma.

Original languageEnglish
Pages (from-to)60872-60884
Number of pages13
JournalOncotarget
Volume7
Issue number38
DOIs
Publication statusPublished - 2016

Keywords

  • AIRE
  • Immune response
  • Immunity
  • Immunology and Microbiology Section
  • MAGE
  • Medullary thymic epithelial cells
  • Single nucleotide polymorphism
  • Tolerance

ASJC Scopus subject areas

  • Oncology

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