Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate

M. Saetta, A. Di Stefano, P. Maestrelli, N. De Marzo, G. F. Milani, F. Pivirotto, C. E. Mapp, L. M. Fabbri

Research output: Contribution to journalArticle

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Abstract

We examined the light and electron microscopic structure of lobar bronchial biopsies of nine subjects with occupational asthma induced by toluene diisocyanate (TDI) and of four control nonasthmatic subjects who had never been exposed to TDI. Inflammatory cell numbers were separately assessed in the intact epithelium, in the more superficial layer of the submucosa, and in the total submucosa. Asthmatic subjects had an increased number of inflammatory cells in the airway mucosa compared with control subjects. Eosinophils were significantly increased in all compartments, CD45-positive cells were significantly increased in the epithelium and in the more superficial layer of the submucosa, and mast cells were significantly increased only in the epithelium. By electron microscopy eosinophils and mast cells appeared degranulated only in asthmatic patients. In the areas of epithelium that appeared intact by light microscopy, electron microscopy showed that, although the intercellular spaces between columnar cells were similar in asthmatic and control groups, the intercellular spaces between basal cells were significantly wider in patients with asthma. Patients with TDI-induced asthma also had a thicker subepithelial reticular layer, where immunohistochemistry showed the presence of collagen III. In conclusion, in patients with asthma induced by TDI, the airway mucosa shows pathologic features, such as inflammatory cell infiltrate and thickening of subepithelial collagen, similar to those described in atopic asthma.

Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalAmerican Review of Respiratory Disease
Volume145
Issue number1
Publication statusPublished - 1992

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Toluene 2,4-Diisocyanate
Occupational Asthma
Asthma
Epithelium
Inflammation
Extracellular Space
Eosinophils
Mast Cells
Electron Microscopy
Mucous Membrane
Collagen
Cell Count
Light
Microscopy
Immunohistochemistry
Electrons
Biopsy
Control Groups

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Saetta, M., Di Stefano, A., Maestrelli, P., De Marzo, N., Milani, G. F., Pivirotto, F., ... Fabbri, L. M. (1992). Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate. American Review of Respiratory Disease, 145(1), 160-168.

Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate. / Saetta, M.; Di Stefano, A.; Maestrelli, P.; De Marzo, N.; Milani, G. F.; Pivirotto, F.; Mapp, C. E.; Fabbri, L. M.

In: American Review of Respiratory Disease, Vol. 145, No. 1, 1992, p. 160-168.

Research output: Contribution to journalArticle

Saetta, M, Di Stefano, A, Maestrelli, P, De Marzo, N, Milani, GF, Pivirotto, F, Mapp, CE & Fabbri, LM 1992, 'Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate', American Review of Respiratory Disease, vol. 145, no. 1, pp. 160-168.
Saetta M, Di Stefano A, Maestrelli P, De Marzo N, Milani GF, Pivirotto F et al. Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate. American Review of Respiratory Disease. 1992;145(1):160-168.
Saetta, M. ; Di Stefano, A. ; Maestrelli, P. ; De Marzo, N. ; Milani, G. F. ; Pivirotto, F. ; Mapp, C. E. ; Fabbri, L. M. / Airway mucosal inflammation in occupational asthma induced by toluene diisocyanate. In: American Review of Respiratory Disease. 1992 ; Vol. 145, No. 1. pp. 160-168.
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