AKT and MAPK signaling in KGF-treated and UVB-exposed human epidermal cells

Lavinia Vittoria Lotti, Sabrina Rotolo, Federica Francescangeli, Luigi Frati, Maria Rosaria Torrisi, Cinzia Marchese

Research output: Contribution to journalArticlepeer-review

Abstract

Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer of differentiation. The MAP kinase and AKT pathways are involved in proliferation and differentiation of many cell types including keratinocytes. We investigated here the role of KGF in modulating AKT and MAPK activity during differentiation of human keratinocytes. Our results show that the mechanisms of action of KGF are dose-dependent and that a sustained activation of the MAPK signaling cascade causes a negative regulation of AKT. We also demostrated increasing expression of KGFR substrates, such as PAK4 during keratinocyte differentiation parallel to the receptor upregulation.

Original languageEnglish
Pages (from-to)633-642
Number of pages10
JournalJournal of Cellular Physiology
Volume212
Issue number3
DOIs
Publication statusPublished - Sep 2007

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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