Akt2 Gene common allelic variants in insulin resistance and the metabolic syndrome

Vittorio Tassi, Angelo Coco, Libera Padovano, Concetta De Bonis, Salvatore De Cosmo, Vincenzo Trischitta

Research output: Contribution to journalArticle

Abstract

Background and aim: Several lines of evidence indicate that glucose homeostasis may be under the control of Akt2 and it can therefore be seen as a candidate gene for human insulin resistance (IR) and related phenotypes. The aim of our study was the identification of Akt2 common allelic variants that might modulate susceptibility to IR and related metabolic abnormalities. Methods and results: The Akt2 gene (exons, 5′ and 3′ regulatory regions) was re-sequenced in samples of 50 blood donors from the Gargano region. Two single nucleotide polymorphisms (SNPs) in 5′ (rs11669332 and rs969531) and two in 3′ (rs2304186 and C1658T) regulatory regions were exploited in an association study using 661 healthy unrelated Caucasian individuals from the same region. Individuals being homozygous for the T allele of rs11669332 (an Akt2 promoter) showed lower systolic blood pressure (p = 0.04), total/HDL cholesterol ratio (p = 0.02) and the metabolic syndrome score (p = 0.04), while carriers of the A allele of rs969531 (in 5′-UTR) showed higher systolic blood pressure (p = 0.027). The association between phenotypic traits and possible haplotypes was tested as well. However, no haplotype affecting the risk of metabolic abnormalities was found. Conclusions: Two variants in 5′ regulatory region of Akt2 gene are associated and may modulate susceptibility to IR and related metabolic abnormalities.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume18
Issue number4
DOIs
Publication statusPublished - May 2008

Fingerprint

Nucleic Acid Regulatory Sequences
Insulin Resistance
Blood Pressure
Haplotypes
Alleles
Genes
5' Untranslated Regions
Blood Donors
HDL Cholesterol
Single Nucleotide Polymorphism
Exons
Homeostasis
Hypertension
Phenotype
Glucose

Keywords

  • Akt2 gene
  • Insulin resistance
  • Metabolic syndrome
  • Type 2 diabetes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Akt2 Gene common allelic variants in insulin resistance and the metabolic syndrome. / Tassi, Vittorio; Coco, Angelo; Padovano, Libera; De Bonis, Concetta; De Cosmo, Salvatore; Trischitta, Vincenzo.

In: Nutrition, Metabolism and Cardiovascular Diseases, Vol. 18, No. 4, 05.2008, p. 263-270.

Research output: Contribution to journalArticle

@article{7dad42c957ff469d8671ee520a400ef9,
title = "Akt2 Gene common allelic variants in insulin resistance and the metabolic syndrome",
abstract = "Background and aim: Several lines of evidence indicate that glucose homeostasis may be under the control of Akt2 and it can therefore be seen as a candidate gene for human insulin resistance (IR) and related phenotypes. The aim of our study was the identification of Akt2 common allelic variants that might modulate susceptibility to IR and related metabolic abnormalities. Methods and results: The Akt2 gene (exons, 5′ and 3′ regulatory regions) was re-sequenced in samples of 50 blood donors from the Gargano region. Two single nucleotide polymorphisms (SNPs) in 5′ (rs11669332 and rs969531) and two in 3′ (rs2304186 and C1658T) regulatory regions were exploited in an association study using 661 healthy unrelated Caucasian individuals from the same region. Individuals being homozygous for the T allele of rs11669332 (an Akt2 promoter) showed lower systolic blood pressure (p = 0.04), total/HDL cholesterol ratio (p = 0.02) and the metabolic syndrome score (p = 0.04), while carriers of the A allele of rs969531 (in 5′-UTR) showed higher systolic blood pressure (p = 0.027). The association between phenotypic traits and possible haplotypes was tested as well. However, no haplotype affecting the risk of metabolic abnormalities was found. Conclusions: Two variants in 5′ regulatory region of Akt2 gene are associated and may modulate susceptibility to IR and related metabolic abnormalities.",
keywords = "Akt2 gene, Insulin resistance, Metabolic syndrome, Type 2 diabetes",
author = "Vittorio Tassi and Angelo Coco and Libera Padovano and {De Bonis}, Concetta and {De Cosmo}, Salvatore and Vincenzo Trischitta",
year = "2008",
month = "5",
doi = "10.1016/j.numecd.2007.01.005",
language = "English",
volume = "18",
pages = "263--270",
journal = "Nutrition, Metabolism and Cardiovascular Diseases",
issn = "0939-4753",
publisher = "Elsevier B.V.",
number = "4",

}

TY - JOUR

T1 - Akt2 Gene common allelic variants in insulin resistance and the metabolic syndrome

AU - Tassi, Vittorio

AU - Coco, Angelo

AU - Padovano, Libera

AU - De Bonis, Concetta

AU - De Cosmo, Salvatore

AU - Trischitta, Vincenzo

PY - 2008/5

Y1 - 2008/5

N2 - Background and aim: Several lines of evidence indicate that glucose homeostasis may be under the control of Akt2 and it can therefore be seen as a candidate gene for human insulin resistance (IR) and related phenotypes. The aim of our study was the identification of Akt2 common allelic variants that might modulate susceptibility to IR and related metabolic abnormalities. Methods and results: The Akt2 gene (exons, 5′ and 3′ regulatory regions) was re-sequenced in samples of 50 blood donors from the Gargano region. Two single nucleotide polymorphisms (SNPs) in 5′ (rs11669332 and rs969531) and two in 3′ (rs2304186 and C1658T) regulatory regions were exploited in an association study using 661 healthy unrelated Caucasian individuals from the same region. Individuals being homozygous for the T allele of rs11669332 (an Akt2 promoter) showed lower systolic blood pressure (p = 0.04), total/HDL cholesterol ratio (p = 0.02) and the metabolic syndrome score (p = 0.04), while carriers of the A allele of rs969531 (in 5′-UTR) showed higher systolic blood pressure (p = 0.027). The association between phenotypic traits and possible haplotypes was tested as well. However, no haplotype affecting the risk of metabolic abnormalities was found. Conclusions: Two variants in 5′ regulatory region of Akt2 gene are associated and may modulate susceptibility to IR and related metabolic abnormalities.

AB - Background and aim: Several lines of evidence indicate that glucose homeostasis may be under the control of Akt2 and it can therefore be seen as a candidate gene for human insulin resistance (IR) and related phenotypes. The aim of our study was the identification of Akt2 common allelic variants that might modulate susceptibility to IR and related metabolic abnormalities. Methods and results: The Akt2 gene (exons, 5′ and 3′ regulatory regions) was re-sequenced in samples of 50 blood donors from the Gargano region. Two single nucleotide polymorphisms (SNPs) in 5′ (rs11669332 and rs969531) and two in 3′ (rs2304186 and C1658T) regulatory regions were exploited in an association study using 661 healthy unrelated Caucasian individuals from the same region. Individuals being homozygous for the T allele of rs11669332 (an Akt2 promoter) showed lower systolic blood pressure (p = 0.04), total/HDL cholesterol ratio (p = 0.02) and the metabolic syndrome score (p = 0.04), while carriers of the A allele of rs969531 (in 5′-UTR) showed higher systolic blood pressure (p = 0.027). The association between phenotypic traits and possible haplotypes was tested as well. However, no haplotype affecting the risk of metabolic abnormalities was found. Conclusions: Two variants in 5′ regulatory region of Akt2 gene are associated and may modulate susceptibility to IR and related metabolic abnormalities.

KW - Akt2 gene

KW - Insulin resistance

KW - Metabolic syndrome

KW - Type 2 diabetes

UR - http://www.scopus.com/inward/record.url?scp=44049098781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44049098781&partnerID=8YFLogxK

U2 - 10.1016/j.numecd.2007.01.005

DO - 10.1016/j.numecd.2007.01.005

M3 - Article

C2 - 17576055

AN - SCOPUS:44049098781

VL - 18

SP - 263

EP - 270

JO - Nutrition, Metabolism and Cardiovascular Diseases

JF - Nutrition, Metabolism and Cardiovascular Diseases

SN - 0939-4753

IS - 4

ER -