Alendronate treatment induces IL-1B expression and apoptosis in glioblastoma cell line

Paola Maura Tricarico, Angeladine Epate, Fulvio Celsi, Sergio Crovella

Research output: Contribution to journalArticle

Abstract

Alendronate (ALD), one among the nitrogen-containing bisphosphonates (NBPs), is currently used for the treatment of many pathological conditions. Unfortunately, although generally tolerated, NBPs treatment has been associated with central nervous system (CNS) adverse outcomes, such as amnesia, hallucinations and visual disturbances. So, we analyzed the effect of ALD treatment in glial cells, the main sources of cholesterol for neurons and principal cells involved in the immunological defense of the brain. We treated a glial cell line (U87-MG) with increasing doses of ALD (0.1, 1, 10, 25, 50 μM) for 48 h, aimed at evaluating the influence of this drug treatment on IL-1B expression, NLRP3 and CASP1 expression, mitochondrial activity and apoptotic cell death. We observed that ALD treatment, at the higher concentrations, induced a significant increase of IL-1B, NLRP3, CASP1 expression, provoked apoptosis and also mitochondrial damage in U87-MG. Considering the reported CNS adverse outcomes of NBPs treatment, our results confirm ALD side-effects on glial cell model.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalInflammopharmacology
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Alendronate
Glioblastoma
Apoptosis
Diphosphonates
Cell Line
Neuroglia
Nitrogen
Central Nervous System
Hallucinations
Cell Death
Cholesterol
Neurons
Brain
Pharmaceutical Preparations

Keywords

  • Alendronate
  • Apoptosis
  • Glial cell line
  • IL-1B expression
  • Nitrogen-containing bisphosphonates
  • NLRP3 inflammasome

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

Cite this

Alendronate treatment induces IL-1B expression and apoptosis in glioblastoma cell line. / Tricarico, Paola Maura; Epate, Angeladine; Celsi, Fulvio; Crovella, Sergio.

In: Inflammopharmacology, Vol. 26, No. 1, 01.02.2018, p. 285-290.

Research output: Contribution to journalArticle

Tricarico, Paola Maura ; Epate, Angeladine ; Celsi, Fulvio ; Crovella, Sergio. / Alendronate treatment induces IL-1B expression and apoptosis in glioblastoma cell line. In: Inflammopharmacology. 2018 ; Vol. 26, No. 1. pp. 285-290.
@article{c284d9711de4473a9a904d7aacf08bbd,
title = "Alendronate treatment induces IL-1B expression and apoptosis in glioblastoma cell line",
abstract = "Alendronate (ALD), one among the nitrogen-containing bisphosphonates (NBPs), is currently used for the treatment of many pathological conditions. Unfortunately, although generally tolerated, NBPs treatment has been associated with central nervous system (CNS) adverse outcomes, such as amnesia, hallucinations and visual disturbances. So, we analyzed the effect of ALD treatment in glial cells, the main sources of cholesterol for neurons and principal cells involved in the immunological defense of the brain. We treated a glial cell line (U87-MG) with increasing doses of ALD (0.1, 1, 10, 25, 50 μM) for 48 h, aimed at evaluating the influence of this drug treatment on IL-1B expression, NLRP3 and CASP1 expression, mitochondrial activity and apoptotic cell death. We observed that ALD treatment, at the higher concentrations, induced a significant increase of IL-1B, NLRP3, CASP1 expression, provoked apoptosis and also mitochondrial damage in U87-MG. Considering the reported CNS adverse outcomes of NBPs treatment, our results confirm ALD side-effects on glial cell model.",
keywords = "Alendronate, Apoptosis, Glial cell line, IL-1B expression, Nitrogen-containing bisphosphonates, NLRP3 inflammasome",
author = "Tricarico, {Paola Maura} and Angeladine Epate and Fulvio Celsi and Sergio Crovella",
year = "2018",
month = "2",
day = "1",
doi = "10.1007/s10787-017-0369-5",
language = "English",
volume = "26",
pages = "285--290",
journal = "Inflammopharmacology",
issn = "0925-4692",
publisher = "Birkhauser Verlag Basel",
number = "1",

}

TY - JOUR

T1 - Alendronate treatment induces IL-1B expression and apoptosis in glioblastoma cell line

AU - Tricarico, Paola Maura

AU - Epate, Angeladine

AU - Celsi, Fulvio

AU - Crovella, Sergio

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Alendronate (ALD), one among the nitrogen-containing bisphosphonates (NBPs), is currently used for the treatment of many pathological conditions. Unfortunately, although generally tolerated, NBPs treatment has been associated with central nervous system (CNS) adverse outcomes, such as amnesia, hallucinations and visual disturbances. So, we analyzed the effect of ALD treatment in glial cells, the main sources of cholesterol for neurons and principal cells involved in the immunological defense of the brain. We treated a glial cell line (U87-MG) with increasing doses of ALD (0.1, 1, 10, 25, 50 μM) for 48 h, aimed at evaluating the influence of this drug treatment on IL-1B expression, NLRP3 and CASP1 expression, mitochondrial activity and apoptotic cell death. We observed that ALD treatment, at the higher concentrations, induced a significant increase of IL-1B, NLRP3, CASP1 expression, provoked apoptosis and also mitochondrial damage in U87-MG. Considering the reported CNS adverse outcomes of NBPs treatment, our results confirm ALD side-effects on glial cell model.

AB - Alendronate (ALD), one among the nitrogen-containing bisphosphonates (NBPs), is currently used for the treatment of many pathological conditions. Unfortunately, although generally tolerated, NBPs treatment has been associated with central nervous system (CNS) adverse outcomes, such as amnesia, hallucinations and visual disturbances. So, we analyzed the effect of ALD treatment in glial cells, the main sources of cholesterol for neurons and principal cells involved in the immunological defense of the brain. We treated a glial cell line (U87-MG) with increasing doses of ALD (0.1, 1, 10, 25, 50 μM) for 48 h, aimed at evaluating the influence of this drug treatment on IL-1B expression, NLRP3 and CASP1 expression, mitochondrial activity and apoptotic cell death. We observed that ALD treatment, at the higher concentrations, induced a significant increase of IL-1B, NLRP3, CASP1 expression, provoked apoptosis and also mitochondrial damage in U87-MG. Considering the reported CNS adverse outcomes of NBPs treatment, our results confirm ALD side-effects on glial cell model.

KW - Alendronate

KW - Apoptosis

KW - Glial cell line

KW - IL-1B expression

KW - Nitrogen-containing bisphosphonates

KW - NLRP3 inflammasome

UR - http://www.scopus.com/inward/record.url?scp=85021221407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021221407&partnerID=8YFLogxK

U2 - 10.1007/s10787-017-0369-5

DO - 10.1007/s10787-017-0369-5

M3 - Article

AN - SCOPUS:85021221407

VL - 26

SP - 285

EP - 290

JO - Inflammopharmacology

JF - Inflammopharmacology

SN - 0925-4692

IS - 1

ER -