Abstract
Leukemic cells spontaneously secrete cytokines involved in the proliferation of the clone; in this study we evaluated the effects of all-trans retinoic acid (ATRA) on the in vitro autocrine production of cytokines by acute myeloid leukemia cells. Thirty acute nonlymphoid leukemia cases (ANLL) (10 APL and 20 ANLL of other cytotypes than APL) were studied; the in vitro secretions of IL-1α, IL-3, IL-4, IL-6, IL-10, G-CSF, GM-CSF, TNF-α were tested with and without ATRA addition. After 5 d exposure to ATRA 10-6 M APL-treated samples showed a significant reduction of IL-6 (p = 0.008) and GM-CSF (p = 0.03) and a significant increase of IL-1α (p = 0.01) production, if compared to untreated APL samples. No difference was seen in IL-3, IL-10 and IL-4 productions; G-CSF production resulted absent in all but 3 APL cases, in which addition of ATRA determined increase in the production. Interestingly, the 3 G-CSF-producing cases did not obtain clinical remission with ATRA; GM-CSF and IL-6 were spontaneously produced by all the cases, and 7 of 10 APL patients subsequently obtained complete remission after induction. TNF-α was produced only in 1 case. No statistical difference was seen in all the productions obtained from other than promyelocytic acute leukemic cells, both with and without ATRA addition. However, it is noteworthy that the production of IL-6 was more than twice as high in ANLL non-APL than in APL cases. In conclusion, these data could thus suggest possible complementary mechanisms of the exhaustion of the leukemic clone upon treatment with ATRA.
| Original language | English |
|---|---|
| Pages (from-to) | 301-306 |
| Number of pages | 6 |
| Journal | European Journal of Haematology |
| Volume | 57 |
| Issue number | 4 |
| Publication status | Published - 1996 |
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Keywords
- colony stimulating factors
- cytokines
- interleukins
- leukemia
- promyelocytic
- retinoids
ASJC Scopus subject areas
- Hematology
Cite this
All-trans retinoic acid and in vitro cytokine production by acute promyelocytic leukemia cells. / Visani, G.; Tosi, P.; Ottaviani, E.; Zaccaria, A.; Baccini, C.; Manfroi, S.; Pastano, R.; Remiddi, C.; Morelli, A.; Molinari, A. L.; Zanchini, R.; Tura, S.
In: European Journal of Haematology, Vol. 57, No. 4, 1996, p. 301-306.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - All-trans retinoic acid and in vitro cytokine production by acute promyelocytic leukemia cells
AU - Visani, G.
AU - Tosi, P.
AU - Ottaviani, E.
AU - Zaccaria, A.
AU - Baccini, C.
AU - Manfroi, S.
AU - Pastano, R.
AU - Remiddi, C.
AU - Morelli, A.
AU - Molinari, A. L.
AU - Zanchini, R.
AU - Tura, S.
PY - 1996
Y1 - 1996
N2 - Leukemic cells spontaneously secrete cytokines involved in the proliferation of the clone; in this study we evaluated the effects of all-trans retinoic acid (ATRA) on the in vitro autocrine production of cytokines by acute myeloid leukemia cells. Thirty acute nonlymphoid leukemia cases (ANLL) (10 APL and 20 ANLL of other cytotypes than APL) were studied; the in vitro secretions of IL-1α, IL-3, IL-4, IL-6, IL-10, G-CSF, GM-CSF, TNF-α were tested with and without ATRA addition. After 5 d exposure to ATRA 10-6 M APL-treated samples showed a significant reduction of IL-6 (p = 0.008) and GM-CSF (p = 0.03) and a significant increase of IL-1α (p = 0.01) production, if compared to untreated APL samples. No difference was seen in IL-3, IL-10 and IL-4 productions; G-CSF production resulted absent in all but 3 APL cases, in which addition of ATRA determined increase in the production. Interestingly, the 3 G-CSF-producing cases did not obtain clinical remission with ATRA; GM-CSF and IL-6 were spontaneously produced by all the cases, and 7 of 10 APL patients subsequently obtained complete remission after induction. TNF-α was produced only in 1 case. No statistical difference was seen in all the productions obtained from other than promyelocytic acute leukemic cells, both with and without ATRA addition. However, it is noteworthy that the production of IL-6 was more than twice as high in ANLL non-APL than in APL cases. In conclusion, these data could thus suggest possible complementary mechanisms of the exhaustion of the leukemic clone upon treatment with ATRA.
AB - Leukemic cells spontaneously secrete cytokines involved in the proliferation of the clone; in this study we evaluated the effects of all-trans retinoic acid (ATRA) on the in vitro autocrine production of cytokines by acute myeloid leukemia cells. Thirty acute nonlymphoid leukemia cases (ANLL) (10 APL and 20 ANLL of other cytotypes than APL) were studied; the in vitro secretions of IL-1α, IL-3, IL-4, IL-6, IL-10, G-CSF, GM-CSF, TNF-α were tested with and without ATRA addition. After 5 d exposure to ATRA 10-6 M APL-treated samples showed a significant reduction of IL-6 (p = 0.008) and GM-CSF (p = 0.03) and a significant increase of IL-1α (p = 0.01) production, if compared to untreated APL samples. No difference was seen in IL-3, IL-10 and IL-4 productions; G-CSF production resulted absent in all but 3 APL cases, in which addition of ATRA determined increase in the production. Interestingly, the 3 G-CSF-producing cases did not obtain clinical remission with ATRA; GM-CSF and IL-6 were spontaneously produced by all the cases, and 7 of 10 APL patients subsequently obtained complete remission after induction. TNF-α was produced only in 1 case. No statistical difference was seen in all the productions obtained from other than promyelocytic acute leukemic cells, both with and without ATRA addition. However, it is noteworthy that the production of IL-6 was more than twice as high in ANLL non-APL than in APL cases. In conclusion, these data could thus suggest possible complementary mechanisms of the exhaustion of the leukemic clone upon treatment with ATRA.
KW - colony stimulating factors
KW - cytokines
KW - interleukins
KW - leukemia
KW - promyelocytic
KW - retinoids
UR - http://www.scopus.com/inward/record.url?scp=10544248174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10544248174&partnerID=8YFLogxK
M3 - Article
C2 - 8982293
AN - SCOPUS:10544248174
VL - 57
SP - 301
EP - 306
JO - European Journal of Haematology
JF - European Journal of Haematology
SN - 0902-4441
IS - 4
ER -