TY - JOUR
T1 - All-trans retinoic acid and induction of apoptosis in acute promyelocytic leukemia cells
AU - Tosi, Patrizia
AU - Visani, Giuseppe
AU - Gibellini, Davide
AU - Zauli, Giorgio
AU - Ottaviani, Emanuela
AU - Cenacchi, Annarita
AU - Gamberi, Barbara
AU - Manfroi, Silvia
AU - Marchisiot, Marco
AU - Tura, Sante
PY - 1994
Y1 - 1994
N2 - All-trans retinoic acid (ATRA) represents a highly effective treatment for acute promyelocytic leukemia (M3-ANLL). This compound induces the leukemic promyelocytes to differentiate into morphologically and phenotypically mature myeloid cells. The mechanism of action of ATRA is far from fully understood. It has recently been reported that, along with its differentiation activity, ATRA causes apoptosis in the acute promyelocytic leukemia cell line HL-60. In this study we attempted to test whether ATRA is also able to induce apoptosis in fresh leukemic cells from M3-ANLL patients. Our results indicated that although morphological differentiation was detectable in 9/9 M3-ANLL samples after in vitro exposure to ATRA 1CT6 M., the percentage of apoptotic cells in the treated samples did not significantly differ from that obtained in controls (13.1% vs 9.4% respectively, after 8 days exposure). These data suggest that apoptosis does not seem to be the key mechanism by which ATRA exerts its action in M3-ANLL, at least at the blast cell level.
AB - All-trans retinoic acid (ATRA) represents a highly effective treatment for acute promyelocytic leukemia (M3-ANLL). This compound induces the leukemic promyelocytes to differentiate into morphologically and phenotypically mature myeloid cells. The mechanism of action of ATRA is far from fully understood. It has recently been reported that, along with its differentiation activity, ATRA causes apoptosis in the acute promyelocytic leukemia cell line HL-60. In this study we attempted to test whether ATRA is also able to induce apoptosis in fresh leukemic cells from M3-ANLL patients. Our results indicated that although morphological differentiation was detectable in 9/9 M3-ANLL samples after in vitro exposure to ATRA 1CT6 M., the percentage of apoptotic cells in the treated samples did not significantly differ from that obtained in controls (13.1% vs 9.4% respectively, after 8 days exposure). These data suggest that apoptosis does not seem to be the key mechanism by which ATRA exerts its action in M3-ANLL, at least at the blast cell level.
KW - Acute promyelocytic leukemia
KW - All-trans retinoic acid
KW - Apoptosis
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U2 - 10.3109/10428199409049711
DO - 10.3109/10428199409049711
M3 - Article
C2 - 7812212
AN - SCOPUS:0028168185
VL - 14
SP - 503
EP - 507
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
SN - 1042-8194
IS - 5-6
ER -