All-trans retinoic acid at low concentration directly stimulates normal adult megakaryocytopoiesis in the presence of thrombopoietin or combined cytokines

Giuseppe Visani, Emanuela Ottaviani, Giorgio Zauli, Patrizia Tosi, Annalisa Pellacani, Alessandro Isidori, Sabina Pierpaoli, Sante Tura

Research output: Contribution to journalArticlepeer-review

Abstract

In order to investigate the direct effects of retinoids on normal adult hematopoietic progenitors, purified CD34+ cells were seeded in serum-free cultures in the presence of pharmacological (10-6) M or physiological (10- 12) M concentrations of all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) plus combinations of specific cytokines. 10-6 M ATRA and 9- cis RA significantly decreased the number of granulomacrophagic, erythroid and megakaryocytic (CFU- meg) progenitors. On the other hand, 10-12 M ATRA significantly promoted the growth of CFU-meg, in the presence either of thrombopoietin or of IL-3+GM-CSF, and induced a reproducible stimulation of the immature CD34+DR- subset. In conclusion, our findings suggest that retinoic acids probably play a direct role in normal adult hematopoietic development at both physiological and pharmacological concentrations. The stimulatory effect on megakaryocytopoiesis should be considered in the perspective of a potential use of low-dose ATRA, combined with thrombopoietin or other cytokines, in pathological conditions where the megakaryocytic compartment is impaired and the stimulation of megakaryocytopoiesis is requested.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalEuropean Journal of Haematology
Volume63
Issue number3
Publication statusPublished - 1999

Keywords

  • All-trans retinoic acid
  • Autolog ous transplantation
  • CD34+
  • Chemotherapy
  • Cytokines
  • Megakaryocytopoiesis
  • Thrombopoietin

ASJC Scopus subject areas

  • Hematology

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