Allelic effects of mouse Pas1 candidate genes in human lung cancer cell lines

Federica Galbiati, Angela Pettinicchio, Tommaso A. Dragani, Giacomo Manenti

Research output: Contribution to journalArticlepeer-review

Abstract

Four of the six genes constituting the mouse Pulmonary adenoma susceptibility 1 (Pas1) locus haplotype carry amino acid variants: Lrmp, Casc1, Ghiso, and Lmna-rs1. In vitro colony formation assay of human lung cancer cell lines A549 and NCI-H520 transfected with the allelic variants of the four genes revealed allele-specific modulations of colony numbers by Lmna-rs1 and Casc1, but not by Lrmp or Ghiso. In A549 and NCI-H520 cells, the A/J allele of Lmna-rs1 produced ∼4- and ∼2-fold, respectively, more transfectants than did the C57BL/6J allele, whereas the A/J allele of Casc1 produced ∼6- and ∼5-fold fewer transfectants, respectively, as compared to the C57BL/6J allele. Inhibition of clonogenicity by allelic forms of Pas1 candidate genes was not mediated by induction of apoptosis. These findings provide evidence that allelic variants of mouse Pas1 candidate genes differentially modulate growth of human cancer cells.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalCancer Letters
Volume244
Issue number2
DOIs
Publication statusPublished - Dec 8 2006

Keywords

  • Colony formation assay
  • Ghiso
  • Lmrp
  • Lung cancer

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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