Allergen-driven HLA-G expression and secretion in peripheral blood mononuclear cells from allergic rhinitis patients

Paola Contini, Francesco Puppo, Giorgio Walter Canonica, Giuseppe Murdaca, Giorgio Ciprandi

Research output: Contribution to journalArticle

Abstract

Background: It has been reported that soluble HLA-G serum levels are increased in patients with pollen-induced allergic rhinitis and decrease after immunotherapy. However, no functional study has been conducted so far. The aim of this study was to evaluate the membrane expression and secretion of HLA-G molecules in peripheral blood mononuclear cells from allergic rhinitis patients after . in vitro incubation with the causal allergen. Methods and results: Twenty-two allergic rhinitis patients and ten healthy subjects were enrolled. Membrane HLA-G expression was determined by flow cytometry and soluble HLA-G in culture supernatant was determined by immunoenzymatic assay. HLA-G expression was detected in CD4+ (T-helper-2) cells and monocytes after . in vitro stimulation with the causal allergen but not with non specific stimuli and non causal allergens. Accordingly, the release of soluble HLA-G in culture supernatant occurred only after the stimulation with the causal allergen. Collectively, these results were confirmed by Western blot analysis. Conclusions: The present study provides the first . in vitro evidence that in allergic patients HLA-G expression and secretion is specifically induced by the causal allergen. These data may add new insights into the pathogenetic mechanisms underlying allergic inflammation and allergen specific immunotherapy.

Original languageEnglish
JournalHuman Immunology
DOIs
Publication statusAccepted/In press - Jul 16 2014

Keywords

  • Allergic rhinitis
  • CD4 T cells
  • HLA-G
  • Monocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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