Allergenic activity of Pseudoterranova decipiens (Nematoda: Anisakidae) in BALB/c mice

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Abstract

Background: Anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. However, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. The aim of this study was to determine if Pseudoterranova decipiens belonging to the family Anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (BALB/c mice) model. Results: The ingestion of A. pegreffii proteins by BALB/c mice, which had been previously sensitized by intraperitoneal inoculation with the corresponding live L3 larvae, triggers signs of allergy within 60 min, whereas P. decipiens did to a lesser extent. Beside symptoms, allergic reactions were furtherly supported by the presence of histamine in sera of sensitized mice. Specific IgG1 and IgE responses were detected in sera of all sensitized mice from week four. Specific IgG2a response was detected in sera from mice sensitized to P. decipiens. After polyclonal or specific activation with anti-CD3/anti-CD28 or antigens, respectively, splenocytes from mice infected i.p. with A. pegreffii or P. decipiens larvae showed significantly higher production of IL-10 than naïve mice. After stimulation with specific antigens, significantly higher IL-5 and IL-13 amounts were produced by specific antigen stimulated splenocytes than by the naïve cells; only P. decipiens proteins induced IFN. Conclusions: The overall results suggest that infection with P. decipiens can sensitize mice to react to subsequent oral challenge with anisakid proteins, as described for A. simplex (sensu stricto) and A. pegreffii infections. The results show that anisakid proteins induce a dominant Th2 response, although P. decipiens could also induce a mixed type 1/type 2 pattern.

Original languageEnglish
Article number290
JournalParasites and Vectors
Volume10
Issue number1
DOIs
Publication statusPublished - Jun 12 2017

Fingerprint

Nematoda
Hypersensitivity
CD28 Antigens
Larva
Proteins
Serum
Anisakis
Antigens
Fisheries
Interleukin-13
Interleukin-5
Infection
Interleukin-10
Allergens
Immunoglobulin E
Histamine
Oral Administration
Parasites
Eating
Immunoglobulin G

Keywords

  • Allergic reactions
  • Anisakidae
  • Anisakis pegreffii
  • BALB/c mice
  • Immune response
  • Pseudoterranova decipiens
  • Sensitization

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

@article{c8090eeec1d14fb48ac7237d149640ca,
title = "Allergenic activity of Pseudoterranova decipiens (Nematoda: Anisakidae) in BALB/c mice",
abstract = "Background: Anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. However, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. The aim of this study was to determine if Pseudoterranova decipiens belonging to the family Anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (BALB/c mice) model. Results: The ingestion of A. pegreffii proteins by BALB/c mice, which had been previously sensitized by intraperitoneal inoculation with the corresponding live L3 larvae, triggers signs of allergy within 60 min, whereas P. decipiens did to a lesser extent. Beside symptoms, allergic reactions were furtherly supported by the presence of histamine in sera of sensitized mice. Specific IgG1 and IgE responses were detected in sera of all sensitized mice from week four. Specific IgG2a response was detected in sera from mice sensitized to P. decipiens. After polyclonal or specific activation with anti-CD3/anti-CD28 or antigens, respectively, splenocytes from mice infected i.p. with A. pegreffii or P. decipiens larvae showed significantly higher production of IL-10 than na{\"i}ve mice. After stimulation with specific antigens, significantly higher IL-5 and IL-13 amounts were produced by specific antigen stimulated splenocytes than by the na{\"i}ve cells; only P. decipiens proteins induced IFN. Conclusions: The overall results suggest that infection with P. decipiens can sensitize mice to react to subsequent oral challenge with anisakid proteins, as described for A. simplex (sensu stricto) and A. pegreffii infections. The results show that anisakid proteins induce a dominant Th2 response, although P. decipiens could also induce a mixed type 1/type 2 pattern.",
keywords = "Allergic reactions, Anisakidae, Anisakis pegreffii, BALB/c mice, Immune response, Pseudoterranova decipiens, Sensitization",
author = "Alessandra Ludovisi and {Di Felice}, Gabriella and Noelia Carballeda-Sangiao and Bianca Barletta and Cinzia Butteroni and Silvia Corinti and Gianluca Marucci and Miguel Gonz{\'a}lez-Mu{\~n}oz and Edoardo Pozio and G{\'o}mez-Morales, {Maria Angeles}",
year = "2017",
month = "6",
day = "12",
doi = "10.1186/s13071-017-2231-4",
language = "English",
volume = "10",
journal = "Parasites and Vectors",
issn = "1756-3305",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Allergenic activity of Pseudoterranova decipiens (Nematoda

T2 - Anisakidae) in BALB/c mice

AU - Ludovisi, Alessandra

AU - Di Felice, Gabriella

AU - Carballeda-Sangiao, Noelia

AU - Barletta, Bianca

AU - Butteroni, Cinzia

AU - Corinti, Silvia

AU - Marucci, Gianluca

AU - González-Muñoz, Miguel

AU - Pozio, Edoardo

AU - Gómez-Morales, Maria Angeles

PY - 2017/6/12

Y1 - 2017/6/12

N2 - Background: Anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. However, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. The aim of this study was to determine if Pseudoterranova decipiens belonging to the family Anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (BALB/c mice) model. Results: The ingestion of A. pegreffii proteins by BALB/c mice, which had been previously sensitized by intraperitoneal inoculation with the corresponding live L3 larvae, triggers signs of allergy within 60 min, whereas P. decipiens did to a lesser extent. Beside symptoms, allergic reactions were furtherly supported by the presence of histamine in sera of sensitized mice. Specific IgG1 and IgE responses were detected in sera of all sensitized mice from week four. Specific IgG2a response was detected in sera from mice sensitized to P. decipiens. After polyclonal or specific activation with anti-CD3/anti-CD28 or antigens, respectively, splenocytes from mice infected i.p. with A. pegreffii or P. decipiens larvae showed significantly higher production of IL-10 than naïve mice. After stimulation with specific antigens, significantly higher IL-5 and IL-13 amounts were produced by specific antigen stimulated splenocytes than by the naïve cells; only P. decipiens proteins induced IFN. Conclusions: The overall results suggest that infection with P. decipiens can sensitize mice to react to subsequent oral challenge with anisakid proteins, as described for A. simplex (sensu stricto) and A. pegreffii infections. The results show that anisakid proteins induce a dominant Th2 response, although P. decipiens could also induce a mixed type 1/type 2 pattern.

AB - Background: Anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. However, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. The aim of this study was to determine if Pseudoterranova decipiens belonging to the family Anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (BALB/c mice) model. Results: The ingestion of A. pegreffii proteins by BALB/c mice, which had been previously sensitized by intraperitoneal inoculation with the corresponding live L3 larvae, triggers signs of allergy within 60 min, whereas P. decipiens did to a lesser extent. Beside symptoms, allergic reactions were furtherly supported by the presence of histamine in sera of sensitized mice. Specific IgG1 and IgE responses were detected in sera of all sensitized mice from week four. Specific IgG2a response was detected in sera from mice sensitized to P. decipiens. After polyclonal or specific activation with anti-CD3/anti-CD28 or antigens, respectively, splenocytes from mice infected i.p. with A. pegreffii or P. decipiens larvae showed significantly higher production of IL-10 than naïve mice. After stimulation with specific antigens, significantly higher IL-5 and IL-13 amounts were produced by specific antigen stimulated splenocytes than by the naïve cells; only P. decipiens proteins induced IFN. Conclusions: The overall results suggest that infection with P. decipiens can sensitize mice to react to subsequent oral challenge with anisakid proteins, as described for A. simplex (sensu stricto) and A. pegreffii infections. The results show that anisakid proteins induce a dominant Th2 response, although P. decipiens could also induce a mixed type 1/type 2 pattern.

KW - Allergic reactions

KW - Anisakidae

KW - Anisakis pegreffii

KW - BALB/c mice

KW - Immune response

KW - Pseudoterranova decipiens

KW - Sensitization

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U2 - 10.1186/s13071-017-2231-4

DO - 10.1186/s13071-017-2231-4

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JO - Parasites and Vectors

JF - Parasites and Vectors

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