Allogeneic gene-modified tumour cells in metastatic kidney cancer. Report II

Giancarlo Pizza, C. De Vinci, G. Lo Conte, A. Mazzuca, V. Di Maio, S. Ratini, G. Severini, L. Busutti, A. P. Palareti, A. Gulino, A. Vacca, L. Melchiorri, M. Ferrari, L. Giacomelli, O. R. Baricordi, S. Forzini, R. Capanna

Research output: Contribution to journalArticle

Abstract

In a limited study, comprising only ten patients, we have previously reported that allogeneic irradiated RCC-cell-line cells, engineered to produce IL-2 (ACHN-IL-2), admixed with autologous metastatic formalin-treated tumour cells were used to vaccinate MRCC patients in progression of disease and also receiving IL-2 immunotherapy. The cells, admixed to autologous TC, were administered subcutaneously. We now report an extended study on thirty patients and one hundred thirty-one controls. Patients received 4-20 injections (mean 10 ± 4), containing an average of 92 × 106 ± 45 × 106 ACHN-IL-2 transfected cells (a minimum of 25 × 106, and a maximum of 200 × 106). Autologous TC, admixed to allogeneic, were also administered by 4-16 s.c. injections (mean 7 ± 3), i.e. a total of 12 × 106-160 × 106 cells. Vaccination was administered during 73-1451 (307 ± 316) days, and the follow-up continued for 1122 ± 1240 days (106-5137). Throughout this period, the patients continued receiving the previously set immunotherapy treatment. No adverse side effects related to the treatment were noticed. One complete and four partial tumour responses were observed, as well as nine cases of stable disease. Thirteen patients died in the treated group (43%) and 63 (44%) in the control group. Responding patients resumed progression in 4-11 months and died 18 and 36 months after beginning the vaccine therapy. The Gehan Wilcoxon's test showed a significantly (P <0.01) better survival in the vaccinated patients compared to that of the controls. Thus, we confirm, in an increased number of patients and an extensive follow-up, that our vaccination protocol is safe, devoid of adverse side effects, and promising.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalFolia Biologica
Volume50
Issue number6
Publication statusPublished - 2004

Keywords

  • Gene-modified
  • IL-2
  • Immunotherapy
  • MRCC
  • Renal cancer
  • Vaccine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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