Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain

Nina Lauterbach, Pietro Crivello, Lotte Wieten, Laura Zito, Mathijs Groeneweg, Christina E M Voorter, Katharina Fleischhauer, Marcel G J Tilanus

Research output: Contribution to journalArticle

Abstract

Alloreactivity to HLA-DP molecules, class II heterodimers of an oligomorphic alpha and a polymorphic beta chain, is increasingly being studied due to its relevance in clinical transplantation. We hypothesized that not only polymorphisms in the peptide binding groove encoded by exon 2 of HLA-DPB1, but also in other regions of the molecule and the alpha chain, could play a role in CD4+ T cell allorecognition. To test this possibility, we comparatively investigated CD4+ T cell allorecognition, measured by upregulation of the activation marker CD137, against HLA-DPB1*13:01, *05:01, *03:01, *17:01 or their allele counter parts DPB1*107:01, *135:01, *104:01, *131:01, with identical exon 2 sequences but polymorphism in exons 1, 3 or 4, in the context of different HLA-DPA1 (DPA1) polymorphisms (DPA1*01:03 and *02:01). No significant differences in CD4+ T cell allorecognition levels could be demonstrated for any of the beyond exon 2 DPB1 variants studied. Interestingly, however, the mean fold change in CD4+ CD137+ cells was significantly higher when the target shared at least one DPA1 allele with the allogeneic stimulator, compared to a distinct DPA1 background (1.65 vs 0.23, P

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalMolecular Immunology
Volume59
Issue number1
DOIs
Publication statusPublished - May 2014

Keywords

  • Allorecognition
  • HLA-DPA1
  • HLA-DPB1

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

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    Lauterbach, N., Crivello, P., Wieten, L., Zito, L., Groeneweg, M., Voorter, C. E. M., Fleischhauer, K., & Tilanus, M. G. J. (2014). Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Molecular Immunology, 59(1), 19-29. https://doi.org/10.1016/j.molimm.2013.12.006