Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma

A. Balsari, G. Fossati, D. Taramelli, G. Tona, D. Delia, R. Giardini, G. Parmiani

Research output: Contribution to journalArticle

Abstract

Killing of autologous melanoma (auto-Me) was obtained with pooled allostimulated peripheral blood lymphocytes (PBL) in 34/42 cases and found not to be due to a cross-reactivity between melanoma and allogeneic normal antigens. To see whether generation of tumour cytotoxic PBL by allostimulation was due to release of IL-2, PBL from 34 patients were divided into two aliquots and stimulated either by alloantigens or IL-2. Allostimulated PBL were cytotoxic for auto-Me in 30/34 cases (85%) whereas IL-2 generated tumour cytotoxic cells in 22/34 cases (64%). Lysis of K562, a target for monitoring NK-like activity, was obtained in 95-100% of cases with both stimuli. A similar frequency of OKT3+, OKT4+, OKT8+ and HNK1+ cells was found in PBL activated by allostimulation and IL-2, whereas a higher frequency of OKM1+ cells was evident in IL-2-stimulated PBL. Cold-target competition studies indicated that allostimulation generated at least two different types of effectors, one lytic to auto-Me but not to K562, and the other which lysed both targets. Allostimulated, FACS-separated T3- cells killed both auto-Me and K562 cells whereas T3+ cells lysed only auto-Me. It is concluded that allostimulation generated two subpopulations of auto-Me killer cells, one of the T lineage and the other NK-like, which both can destroy auto-Me targets.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalBritish Journal of Cancer
Volume52
Issue number1
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Balsari, A., Fossati, G., Taramelli, D., Tona, G., Delia, D., Giardini, R., & Parmiani, G. (1985). Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma. British Journal of Cancer, 52(1), 73-80. https://doi.org/10.1038/bjc.1985.151