Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma

A. Balsari, G. Fossati, D. Taramelli, G. Tona, D. Delia, R. Giardini, G. Parmiani

Research output: Contribution to journalArticle

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Abstract

Killing of autologous melanoma (auto-Me) was obtained with pooled allostimulated peripheral blood lymphocytes (PBL) in 34/42 cases and found not to be due to a cross-reactivity between melanoma and allogeneic normal antigens. To see whether generation of tumour cytotoxic PBL by allostimulation was due to release of IL-2, PBL from 34 patients were divided into two aliquots and stimulated either by alloantigens or IL-2. Allostimulated PBL were cytotoxic for auto-Me in 30/34 cases (85%) whereas IL-2 generated tumour cytotoxic cells in 22/34 cases (64%). Lysis of K562, a target for monitoring NK-like activity, was obtained in 95-100% of cases with both stimuli. A similar frequency of OKT3+, OKT4+, OKT8+ and HNK1+ cells was found in PBL activated by allostimulation and IL-2, whereas a higher frequency of OKM1+ cells was evident in IL-2-stimulated PBL. Cold-target competition studies indicated that allostimulation generated at least two different types of effectors, one lytic to auto-Me but not to K562, and the other which lysed both targets. Allostimulated, FACS-separated T3- cells killed both auto-Me and K562 cells whereas T3+ cells lysed only auto-Me. It is concluded that allostimulation generated two subpopulations of auto-Me killer cells, one of the T lineage and the other NK-like, which both can destroy auto-Me targets.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalBritish Journal of Cancer
Volume52
Issue number1
DOIs
Publication statusPublished - 1985

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Natural Killer Cells
Melanoma
Lymphocytes
Interleukin-2
Muromonab-CD3
Isoantigens
K562 Cells
Neoplasms
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Balsari, A., Fossati, G., Taramelli, D., Tona, G., Delia, D., Giardini, R., & Parmiani, G. (1985). Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma. British Journal of Cancer, 52(1), 73-80. https://doi.org/10.1038/bjc.1985.151

Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma. / Balsari, A.; Fossati, G.; Taramelli, D.; Tona, G.; Delia, D.; Giardini, R.; Parmiani, G.

In: British Journal of Cancer, Vol. 52, No. 1, 1985, p. 73-80.

Research output: Contribution to journalArticle

Balsari, A, Fossati, G, Taramelli, D, Tona, G, Delia, D, Giardini, R & Parmiani, G 1985, 'Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma', British Journal of Cancer, vol. 52, no. 1, pp. 73-80. https://doi.org/10.1038/bjc.1985.151
Balsari, A. ; Fossati, G. ; Taramelli, D. ; Tona, G. ; Delia, D. ; Giardini, R. ; Parmiani, G. / Allostimulation of patients' lymphocytes generates both T and NK-like cells cytotoxic for autologous melanoma. In: British Journal of Cancer. 1985 ; Vol. 52, No. 1. pp. 73-80.
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