ALMS1-deficient fibroblasts over-express extra-cellular matrix components, display cell cycle delay and are resistant to apoptosis

Elisabetta Zulato, Francesca Favaretto, Caterina Veronese, Stefano Campanaro, Jan D. Marshall, Sara Romano, Anna Cabrelle, Gayle B. Collin, Barbara Zavan, Anna S. Belloni, Enrica Rampazzo, Jürgen K. Naggert, Giovanni Abatangelo, Nicola Sicolo, Pietro Maffei, Gabriella Milan, Roberto Vettor

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Alström Syndrome (ALMS) is a rare genetic disorder (483 living cases), characterized by many clinical manifestations, including blindness, obesity, type 2 diabetes and cardiomyopathy. ALMS is caused by mutations in the ALMS1 gene, encoding for a large protein with implicated roles in ciliary function, cellular quiescence and intracellular transport. Patients with ALMS have extensive fibrosis in nearly all tissues resulting in a progressive organ failure which is often the ultimate cause of death. To focus on the role of ALMS1 mutations in the generation and maintenance of this pathological fibrosis, we performed gene expression analysis, ultrastructural characterization and functional assays in 4 dermal fibroblast cultures from ALMS patients. Using a genome-wide gene expression analysis we found alterations in genes belonging to specific categories (cell cycle, extracellular matrix (ECM) and fibrosis, cellular architecture/motility and apoptosis). ALMS fibroblasts display cytoskeleton abnormalities and migration impairment, up-regulate the expression and production of collagens and despite the increase in the cell cycle length are more resistant to apoptosis. Therefore ALMS1-deficient fibroblasts showed a constitutively activated myofibroblast phenotype even if they do not derive from a fibrotic lesion. Our results support a genetic basis for the fibrosis observed in ALMS and show that both an excessive ECM production and a failure to eliminate myofibroblasts are key mechanisms. Furthermore, our findings suggest new roles for ALMS1 in both intra- and extra-cellular events which are essential not only for the normal cellular function but also for cell-cell and ECM-cell interactions.

Original languageEnglish
Article numbere19081
JournalPLoS One
Volume6
Issue number4
DOIs
Publication statusPublished - 2011

Fingerprint

Fibroblasts
fibrosis
extracellular matrix
fibroblasts
cell cycle
Cell Cycle
apoptosis
Display devices
Cells
Apoptosis
Gene expression
Fibrosis
Genes
Extracellular Matrix
Gene encoding
Myofibroblasts
Medical problems
mutation
Cell culture
gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

ALMS1-deficient fibroblasts over-express extra-cellular matrix components, display cell cycle delay and are resistant to apoptosis. / Zulato, Elisabetta; Favaretto, Francesca; Veronese, Caterina; Campanaro, Stefano; Marshall, Jan D.; Romano, Sara; Cabrelle, Anna; Collin, Gayle B.; Zavan, Barbara; Belloni, Anna S.; Rampazzo, Enrica; Naggert, Jürgen K.; Abatangelo, Giovanni; Sicolo, Nicola; Maffei, Pietro; Milan, Gabriella; Vettor, Roberto.

In: PLoS One, Vol. 6, No. 4, e19081, 2011.

Research output: Contribution to journalArticle

Zulato, E, Favaretto, F, Veronese, C, Campanaro, S, Marshall, JD, Romano, S, Cabrelle, A, Collin, GB, Zavan, B, Belloni, AS, Rampazzo, E, Naggert, JK, Abatangelo, G, Sicolo, N, Maffei, P, Milan, G & Vettor, R 2011, 'ALMS1-deficient fibroblasts over-express extra-cellular matrix components, display cell cycle delay and are resistant to apoptosis', PLoS One, vol. 6, no. 4, e19081. https://doi.org/10.1371/journal.pone.0019081
Zulato, Elisabetta ; Favaretto, Francesca ; Veronese, Caterina ; Campanaro, Stefano ; Marshall, Jan D. ; Romano, Sara ; Cabrelle, Anna ; Collin, Gayle B. ; Zavan, Barbara ; Belloni, Anna S. ; Rampazzo, Enrica ; Naggert, Jürgen K. ; Abatangelo, Giovanni ; Sicolo, Nicola ; Maffei, Pietro ; Milan, Gabriella ; Vettor, Roberto. / ALMS1-deficient fibroblasts over-express extra-cellular matrix components, display cell cycle delay and are resistant to apoptosis. In: PLoS One. 2011 ; Vol. 6, No. 4.
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AU - Zulato, Elisabetta

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AU - Campanaro, Stefano

AU - Marshall, Jan D.

AU - Romano, Sara

AU - Cabrelle, Anna

AU - Collin, Gayle B.

AU - Zavan, Barbara

AU - Belloni, Anna S.

AU - Rampazzo, Enrica

AU - Naggert, Jürgen K.

AU - Abatangelo, Giovanni

AU - Sicolo, Nicola

AU - Maffei, Pietro

AU - Milan, Gabriella

AU - Vettor, Roberto

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