alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies

Matilde Bongianni, Anna Ladogana, Stefano Capaldi, Sigrid Klotz, Simone Baiardi, Annachiara Cagnin, Daniela Perra, Michele Fiorini, Anna Poleggi, Giuseppe Legname, Tatiana Cattaruzza, Francesco Janes, Massimo Tabaton, Bernardino Ghetti, Salvatore Monaco, Gabor G. Kovacs, Piero Parchi, Maurizio Pocchiari, Gianluigi Zanusso

Research output: Contribution to journalArticlepeer-review


We applied RT-QuIC assay to detect alpha-synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt-Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with alpha-synuclein mixed pathology (n = 20), or without Lewy-related pathology (n = 49). The test had a sensitivity of 92.95.9synucleinopathies from non-alpha-synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT-QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT-QuIC for alpha-synuclein is an accurate test for DLB diagnosis.
Original languageEnglish
Pages (from-to)2120-2126
Number of pages7
JournalAnnals of Clinical and Translational Neurology
Issue number10
Publication statusPublished - Oct 1 2019


Dive into the research topics of 'alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies'. Together they form a unique fingerprint.

Cite this