alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies

Matilde Bongianni; Anna Ladogana; Stefano Capaldi; Sigrid Klotz; Simone Baiardi; Annachiara Cagnin; Daniela Perra; Michele Fiorini; Anna Poleggi; Giuseppe Legname; Tatiana Cattaruzza; Francesco Janes; Massimo Tabaton; Bernardino Ghetti; Salvatore Monaco; Gabor G. Kovacs; Piero Parchi; Maurizio Pocchiari; Gianluigi Zanusso

doi:10.1002/acn3.50897

alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies

Matilde Bongianni, Anna Ladogana, Stefano Capaldi, Sigrid Klotz, Simone Baiardi, Annachiara Cagnin, Daniela Perra, Michele Fiorini, Anna Poleggi, Giuseppe Legname, Tatiana Cattaruzza, Francesco Janes, Massimo Tabaton, Bernardino Ghetti, Salvatore Monaco, Gabor G. Kovacs, Piero Parchi, Maurizio Pocchiari, Gianluigi Zanusso

Research output: Contribution to journal › Article › peer-review

Abstract

We applied RT-QuIC assay to detect alpha-synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt-Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with alpha-synuclein mixed pathology (n = 20), or without Lewy-related pathology (n = 49). The test had a sensitivity of 92.95.9synucleinopathies from non-alpha-synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT-QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT-QuIC for alpha-synuclein is an accurate test for DLB diagnosis.

Original language	English
Pages (from-to)	2120-2126
Number of pages	7
Journal	Annals of Clinical and Translational Neurology
Volume	6
Issue number	10
DOIs	https://doi.org/10.1002/acn3.50897
Publication status	Published - Oct 1 2019

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Bongianni, M., Ladogana, A., Capaldi, S., Klotz, S., Baiardi, S., Cagnin, A., Perra, D., Fiorini, M., Poleggi, A., Legname, G., Cattaruzza, T., Janes, F., Tabaton, M., Ghetti, B., Monaco, S., Kovacs, G. G., Parchi, P., Pocchiari, M., & Zanusso, G. (2019). alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. Annals of Clinical and Translational Neurology, 6(10), 2120-2126. https://doi.org/10.1002/acn3.50897

alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. / Bongianni, Matilde; Ladogana, Anna; Capaldi, Stefano; Klotz, Sigrid; Baiardi, Simone; Cagnin, Annachiara; Perra, Daniela; Fiorini, Michele; Poleggi, Anna; Legname, Giuseppe; Cattaruzza, Tatiana; Janes, Francesco; Tabaton, Massimo; Ghetti, Bernardino; Monaco, Salvatore; Kovacs, Gabor G.; Parchi, Piero ; Pocchiari, Maurizio; Zanusso, Gianluigi.

In: Annals of Clinical and Translational Neurology, Vol. 6, No. 10, 01.10.2019, p. 2120-2126.

Research output: Contribution to journal › Article › peer-review

Bongianni, M, Ladogana, A, Capaldi, S, Klotz, S, Baiardi, S, Cagnin, A, Perra, D, Fiorini, M, Poleggi, A, Legname, G, Cattaruzza, T, Janes, F, Tabaton, M, Ghetti, B, Monaco, S, Kovacs, GG, Parchi, P , Pocchiari, M & Zanusso, G 2019, 'alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies', Annals of Clinical and Translational Neurology, vol. 6, no. 10, pp. 2120-2126. https://doi.org/10.1002/acn3.50897

Bongianni, Matilde ; Ladogana, Anna ; Capaldi, Stefano ; Klotz, Sigrid ; Baiardi, Simone ; Cagnin, Annachiara ; Perra, Daniela ; Fiorini, Michele ; Poleggi, Anna ; Legname, Giuseppe ; Cattaruzza, Tatiana ; Janes, Francesco ; Tabaton, Massimo ; Ghetti, Bernardino ; Monaco, Salvatore ; Kovacs, Gabor G. ; Parchi, Piero ; Pocchiari, Maurizio ; Zanusso, Gianluigi. / alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. In: Annals of Clinical and Translational Neurology. 2019 ; Vol. 6, No. 10. pp. 2120-2126.

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title = "alpha-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies",

abstract = "We applied RT-QuIC assay to detect alpha-synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt-Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with alpha-synuclein mixed pathology (n = 20), or without Lewy-related pathology (n = 49). The test had a sensitivity of 92.95.9synucleinopathies from non-alpha-synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT-QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT-QuIC for alpha-synuclein is an accurate test for DLB diagnosis.",

author = "Matilde Bongianni and Anna Ladogana and Stefano Capaldi and Sigrid Klotz and Simone Baiardi and Annachiara Cagnin and Daniela Perra and Michele Fiorini and Anna Poleggi and Giuseppe Legname and Tatiana Cattaruzza and Francesco Janes and Massimo Tabaton and Bernardino Ghetti and Salvatore Monaco and Kovacs, {Gabor G.} and Piero Parchi and Maurizio Pocchiari and Gianluigi Zanusso",

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AU - Bongianni, Matilde

AU - Ladogana, Anna

AU - Capaldi, Stefano

AU - Klotz, Sigrid

AU - Baiardi, Simone

AU - Cagnin, Annachiara

AU - Perra, Daniela

AU - Fiorini, Michele

AU - Poleggi, Anna

AU - Legname, Giuseppe

AU - Cattaruzza, Tatiana

AU - Janes, Francesco

AU - Tabaton, Massimo

AU - Ghetti, Bernardino

AU - Monaco, Salvatore

AU - Kovacs, Gabor G.

AU - Parchi, Piero

AU - Pocchiari, Maurizio

AU - Zanusso, Gianluigi

PY - 2019/10/1

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