Alpha1-acid glycoprotein post-translational modifications: A comparative two dimensional electrophoresis based analysis

R. Fortin, F. Ceciliani, P. Sartorelli, A. Miranda-Ribera, L. Musante, G. Candiano, G. F. Greppi, Paola Roncada

Research output: Contribution to journalArticlepeer-review

Abstract

Alpha1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma. A proteomic approach based on two dimensional electrophoresis, immunoblotting and staining of 2DE gels with dyes specific for post-translational modifications (PTMs) such as glycosylation and phosphorylation has been used to evaluate the differential interspecific protein expression of AGP purified from human, bovine and ovine sera. By means of these techniques, several isoforms have been identified in the investigated species: they have been found to change both with regard to the number of isoforms expressed under physiological condition and with regard to the quality of PTMs (i.e. different oligosaccharidic chains, presence/absence of phosphorilations). In particular, it is suggested that bovine serum AGP may have one of the most complex pattern of PTMs among serum proteins of mammals studied so far.

Original languageEnglish
Pages (from-to)430-432
Number of pages3
JournalItalian Journal of Animal Science
Volume6
Issue numberSUPPL. 1
Publication statusPublished - 2007

Keywords

  • Fluorescent staining
  • Immunoblotting
  • Post-translational modifications (PMTs)
  • Two-dimensional gel electrophoresis (2-DE)

ASJC Scopus subject areas

  • Animal Science and Zoology

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