Alpha1-adrenoceptor-mediated excitation of substantia nigra pars reticulata neurons

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Abstract

The effect of noradrenaline was studied in principal neurons of the substantia nigra pars reticulata in rat brain slices using patch clamp recordings. Perfusion of noradrenaline or the α1-adrenoceptor agonist phenylephrine increased the spontaneous firing activity of reticulata cells. The α1-adrenoceptor antagonist prazosin counteracted the effects of noradrenaline. In contrast, the β-adrenoceptor agonist isoproterenol did not affect the activity of reticulata cells and the β-adrenoceptor antagonist pindolol did not prevent noradrenaline's effect. In whole-cell recordings, at -60 mV holding potential, noradrenaline caused a tetrodotoxin-resistant inward current with a time-course similar to the increase in firing activity. Analysis of the reversal potential of this current did not give homogeneous results. The net noradrenaline current could be associated with a conductance decrease or increase, or in some cases it did not reverse over a range from - 120 to -30 mV. It is suggested that noradrenaline increases the excitability of substantia nigra reticulata cells through α1-adrenoceptors. Both a reduction and an increase in membrane conductance may mediate this effect. The increase in the tonic firing of principal reticulata cells caused by noradrenaline may have significant consequences in regulating the final output of the basal ganglia and consequently in motor-related behaviours. (C) 2000 IBRO.

Original languageEnglish
Pages (from-to)599-604
Number of pages6
JournalNeuroscience
Volume98
Issue number3
DOIs
Publication statusPublished - Jun 2000

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Adrenergic Receptors
Norepinephrine
Neurons
Pindolol
Pars Reticulata
Prazosin
Tetrodotoxin
Phenylephrine
Patch-Clamp Techniques
Basal Ganglia
Isoproterenol
Perfusion
Membranes
Brain

Keywords

  • Basal ganglia
  • Noradrenaline
  • Phenylephrine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alpha1-adrenoceptor-mediated excitation of substantia nigra pars reticulata neurons. / Berretta, N.; Bernardi, G.; Mercuri, N. B.

In: Neuroscience, Vol. 98, No. 3, 06.2000, p. 599-604.

Research output: Contribution to journalArticle

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