Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice

S. Garagna, M. Zuccotti, A. Thornhill, R. Fernandez-Donoso, S. Berrios, E. Capanna, C. A. Redi

Research output: Contribution to journalArticle

Abstract

The mammalian cell nucleus consists of numerous compartments involved in the regular unfolding of processes such as DNA replication and transcription, RNA maturation, protein synthesis and cell division. Knowledge is increasing of the relationships between high-order levels of chromatin organization and its spatial organization, and of how these relationships contribute to the various functions carried out in the nucleus. We have studied the spatial arrangement of mouse telocentric chromosomes 5, 11, 13, 15, 16 and 17, some of their metacentric Robertsonian derivatives, and X and Y chromosomes by whole chromosome painting in male germ (spermatogonia, pachytene spermatocytes and spermatids) and Sertoli cells of homozygous and heterozygous individuals. Using dual-colour fluorescence in situ hybridization we found that these chromosomes occupy specific nuclear territories in each cell type analysed. When chromosomes are present as Robertsonian metacentrics in the heterozygous state, that is, as Robertsonian metacentrics and their homologous telocentrics, differences in their nuclear positions are detectable: heterozygosity regularly produces a change in the nuclear position of one of the two homologous telocentrics in all the cell types studied. In the Robertsonian heterozygotes, the vast majority of the Sertoli cells show the sex chromosomes in a condensed state, whereas they appear decondensed in the Robertsonian homozygotes. As the Robertsonian heterozygosities we studied produce a chromosomally derived impairment of male germ-cell differentiation, we discuss the possibility that changes in chromosome spatial territories may alter some nuclear machinery (e.g., synapsis, differential gene expression) important for the correct unfolding of the meiotic process and for the proper functioning of Sertoli cells.

Original languageEnglish
Pages (from-to)4429-4434
Number of pages6
JournalJournal of Cell Science
Volume114
Issue number24
Publication statusPublished - 2001

Fingerprint

Sertoli Cells
Germ Cells
Chromosomes
Chromosome Painting
Chromosome Pairing
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 11
Spermatogonia
Sex Chromosomes
Spermatocytes
Spermatids
Y Chromosome
Homozygote
X Chromosome
Heterozygote
Cell Nucleus
Fluorescence In Situ Hybridization
DNA Replication
Cell Division
Chromatin

Keywords

  • Chromosome translocation
  • Germ cells
  • Nuclear architecture
  • Sertoli cell
  • Subfertile mice
  • X and Y chromosomes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Garagna, S., Zuccotti, M., Thornhill, A., Fernandez-Donoso, R., Berrios, S., Capanna, E., & Redi, C. A. (2001). Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice. Journal of Cell Science, 114(24), 4429-4434.

Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice. / Garagna, S.; Zuccotti, M.; Thornhill, A.; Fernandez-Donoso, R.; Berrios, S.; Capanna, E.; Redi, C. A.

In: Journal of Cell Science, Vol. 114, No. 24, 2001, p. 4429-4434.

Research output: Contribution to journalArticle

Garagna, S, Zuccotti, M, Thornhill, A, Fernandez-Donoso, R, Berrios, S, Capanna, E & Redi, CA 2001, 'Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice', Journal of Cell Science, vol. 114, no. 24, pp. 4429-4434.
Garagna S, Zuccotti M, Thornhill A, Fernandez-Donoso R, Berrios S, Capanna E et al. Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice. Journal of Cell Science. 2001;114(24):4429-4434.
Garagna, S. ; Zuccotti, M. ; Thornhill, A. ; Fernandez-Donoso, R. ; Berrios, S. ; Capanna, E. ; Redi, C. A. / Alteration of nuclear architecture in male germ cells of chromosomally derived subfertile mice. In: Journal of Cell Science. 2001 ; Vol. 114, No. 24. pp. 4429-4434.
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