Alteration of the actin-based cytoskeleton by rabies virus

Pierre Emmanuel Ceccaldi, Flavia Valtorta, Sandrine Braud, Raymond Hellio, Henri Tsiang

Research output: Contribution to journalArticle

Abstract

We investigated the effect of rabies virus infection on the actin cytoskeleton using various techniques. Confocal microscopic examination of rabies virus-infected neuroblastoma cells at late stages of infection revealed a dramatic decrease in F-actin staining. The results of a fluorimetric assay with pyrenylated actin indicated that purified rabies virus nucleocapsid has no direct action on the kinetics of actin polymerization and only a weak effect on the final extent of polymerization. Videomicroscopy experiments with purified components showed that rabies virus nucleocapsid inhibits the actin-bundling effect induced by dephospho-synapsin I, a neuron-specific protein which is known to exert a control on the actin-based cytoskeleton. Thus, the observed decrease in F-actin staining in infected cells might be ascribed to an indirect action of rabies nucleocapsid on the effects of actin-binding proteins such as synapsin I.

Original languageEnglish
Pages (from-to)2831-2835
Number of pages5
JournalJournal of General Virology
Volume78
Issue number11
Publication statusPublished - Nov 1997

Fingerprint

Rabies virus
Actin Cytoskeleton
Actins
Nucleocapsid
Synapsins
Polymerization
Staining and Labeling
Microfilament Proteins
Video Microscopy
Rabies
Virus Diseases
Neuroblastoma
Neurons
Infection
Proteins

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Ceccaldi, P. E., Valtorta, F., Braud, S., Hellio, R., & Tsiang, H. (1997). Alteration of the actin-based cytoskeleton by rabies virus. Journal of General Virology, 78(11), 2831-2835.

Alteration of the actin-based cytoskeleton by rabies virus. / Ceccaldi, Pierre Emmanuel; Valtorta, Flavia; Braud, Sandrine; Hellio, Raymond; Tsiang, Henri.

In: Journal of General Virology, Vol. 78, No. 11, 11.1997, p. 2831-2835.

Research output: Contribution to journalArticle

Ceccaldi, PE, Valtorta, F, Braud, S, Hellio, R & Tsiang, H 1997, 'Alteration of the actin-based cytoskeleton by rabies virus', Journal of General Virology, vol. 78, no. 11, pp. 2831-2835.
Ceccaldi PE, Valtorta F, Braud S, Hellio R, Tsiang H. Alteration of the actin-based cytoskeleton by rabies virus. Journal of General Virology. 1997 Nov;78(11):2831-2835.
Ceccaldi, Pierre Emmanuel ; Valtorta, Flavia ; Braud, Sandrine ; Hellio, Raymond ; Tsiang, Henri. / Alteration of the actin-based cytoskeleton by rabies virus. In: Journal of General Virology. 1997 ; Vol. 78, No. 11. pp. 2831-2835.
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