Alteration of the passive electrical properties of lymphocyte membranes induced by GM1 and GM3 glycolipids

C. Cametti, F. De Luca, A. D'Ilario, M. A. Macrì, B. Maraviglia, F. Bordi, L. Lenti, R. Misasi, M. Sorice

Research output: Contribution to journalArticle

Abstract

The electrical conductivity of normal human lymphocyte suspensions has been measured in the frequency range from 10 kHz to 100 MHz, where a well-pronounced conductivity dispersion occurs, caused by the surface polarization at the interface between the cell membrane and the extracellular solution. We have investigated the alteration of the passive electrical properties of the cytoplasmic cell membrane induced by two different gangliosides (GM1 and GM3) inserted, at various concentrations, into the outer leaflet of membrane double layer. The alterations observed in the dielectric parameters (the membrane conductivity and the membrane permittivity) derived on the basis of a 'double-shell' model, result in an overall increase of the ion permeation across the membrane and an enhanced polarizability of its hydrophilic region for both gangliosides investigated. The relevance of these alterations is discussed.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalBBA - Biomembranes
Volume1111
Issue number2
DOIs
Publication statusPublished - Nov 9 1992

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Keywords

  • Conductance
  • Ganglioside
  • GM1
  • GM3
  • Lymphocyte
  • Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Cametti, C., De Luca, F., D'Ilario, A., Macrì, M. A., Maraviglia, B., Bordi, F., Lenti, L., Misasi, R., & Sorice, M. (1992). Alteration of the passive electrical properties of lymphocyte membranes induced by GM1 and GM3 glycolipids. BBA - Biomembranes, 1111(2), 197-203. https://doi.org/10.1016/0005-2736(92)90311-9