Abstract
The electrical conductivity of normal human lymphocyte suspensions has been measured in the frequency range from 10 kHz to 100 MHz, where a well-pronounced conductivity dispersion occurs, caused by the surface polarization at the interface between the cell membrane and the extracellular solution. We have investigated the alteration of the passive electrical properties of the cytoplasmic cell membrane induced by two different gangliosides (GM1 and GM3) inserted, at various concentrations, into the outer leaflet of membrane double layer. The alterations observed in the dielectric parameters (the membrane conductivity and the membrane permittivity) derived on the basis of a 'double-shell' model, result in an overall increase of the ion permeation across the membrane and an enhanced polarizability of its hydrophilic region for both gangliosides investigated. The relevance of these alterations is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 197-203 |
| Number of pages | 7 |
| Journal | BBA - Biomembranes |
| Volume | 1111 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 9 1992 |
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Keywords
- Conductance
- Ganglioside
- GM1
- GM3
- Lymphocyte
- Membrane
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
Cite this
Alteration of the passive electrical properties of lymphocyte membranes induced by GM1 and GM3 glycolipids. / Cametti, C.; De Luca, F.; D'Ilario, A.; Macrì, M. A.; Maraviglia, B.; Bordi, F.; Lenti, L.; Misasi, R.; Sorice, M.
In: BBA - Biomembranes, Vol. 1111, No. 2, 09.11.1992, p. 197-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alteration of the passive electrical properties of lymphocyte membranes induced by GM1 and GM3 glycolipids
AU - Cametti, C.
AU - De Luca, F.
AU - D'Ilario, A.
AU - Macrì, M. A.
AU - Maraviglia, B.
AU - Bordi, F.
AU - Lenti, L.
AU - Misasi, R.
AU - Sorice, M.
PY - 1992/11/9
Y1 - 1992/11/9
N2 - The electrical conductivity of normal human lymphocyte suspensions has been measured in the frequency range from 10 kHz to 100 MHz, where a well-pronounced conductivity dispersion occurs, caused by the surface polarization at the interface between the cell membrane and the extracellular solution. We have investigated the alteration of the passive electrical properties of the cytoplasmic cell membrane induced by two different gangliosides (GM1 and GM3) inserted, at various concentrations, into the outer leaflet of membrane double layer. The alterations observed in the dielectric parameters (the membrane conductivity and the membrane permittivity) derived on the basis of a 'double-shell' model, result in an overall increase of the ion permeation across the membrane and an enhanced polarizability of its hydrophilic region for both gangliosides investigated. The relevance of these alterations is discussed.
AB - The electrical conductivity of normal human lymphocyte suspensions has been measured in the frequency range from 10 kHz to 100 MHz, where a well-pronounced conductivity dispersion occurs, caused by the surface polarization at the interface between the cell membrane and the extracellular solution. We have investigated the alteration of the passive electrical properties of the cytoplasmic cell membrane induced by two different gangliosides (GM1 and GM3) inserted, at various concentrations, into the outer leaflet of membrane double layer. The alterations observed in the dielectric parameters (the membrane conductivity and the membrane permittivity) derived on the basis of a 'double-shell' model, result in an overall increase of the ion permeation across the membrane and an enhanced polarizability of its hydrophilic region for both gangliosides investigated. The relevance of these alterations is discussed.
KW - Conductance
KW - Ganglioside
KW - GM1
KW - GM3
KW - Lymphocyte
KW - Membrane
UR - http://www.scopus.com/inward/record.url?scp=0026457060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026457060&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(92)90311-9
DO - 10.1016/0005-2736(92)90311-9
M3 - Article
VL - 1111
SP - 197
EP - 203
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -