Alterations of hepatic ATP homeostasis and respiratory chain during development of non-alcoholic steatohepatitis in a rodent model

G. Serviddio, F. Bellanti, R. Tamborra, T. Rollo, A. D. Romano, A. M. Giudetti, N. Capitanio, A. Petrella, G. Vendemiale, E. Altomare

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Mitochondrial dysfunction is considered a key player in non-alcoholic steatohepatitis (NASH) but no data are available on the mitochondrial function and ATP homeostasis in the liver during NASH progression. In the present paper we evaluated the hepatic mitochondrial respiratory chain activity and ATP synthesis in a rodent model of NASH development. Materials and methods: Male Wistar rats fed a High Fat/Methionine-Choline Deficient (MCD) diet to induce NASH or a control diet (SHAM), and sacrificed after 3, 7 and 11 weeks. The oxidative phosphorylation, the F0F1ATPase (ATP synthase) and the ATP content were assessed in liver mitochondria. Results: NASH mitochondria exhibited an increased rate of substrate oxidation at 3 weeks, which returned to below the normal level at 7 and 11 weeks, concomitantly with the coupling between the phosphorylation activity and the mitochondrial respiration (ADP/O). Uncoupling of NASH liver mitochondria did not allow the recovery of the maximal respiration rate at 7 and 11 weeks. The ATPase (ATP synthase) activity was similar in NASH and SHAM rats, but the mitochondrial ATP content was significantly lower in NASH livers. Conclusions: The loss of hepatic ATP stores is not dependent on the F0F1-ATPase but resides in the respiratory chain. Dysfunction of both Complex I and II of the mitochondrial respiratory chain during NASH development implies a mitochondrial adaptive mechanism occurring in the early stages of NASH.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume38
Issue number4
DOIs
Publication statusPublished - Apr 2008

Keywords

  • ATP homeostasis
  • Mitochondria
  • NASH development
  • Oxidative phosphorylation

ASJC Scopus subject areas

  • Medicine(all)

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