Alterations of neocortical neuronal responses to acetylcholine and gaba in rats born to alcohol-dependent mothers

L. Janiri, G. Gobbi, A. M. Persico, M. Santarelli, D. Minciacchi, E. Tempesta

Research output: Contribution to journalArticle

Abstract

Alcohol is known to be a CNS teratogenic factor interfering with neuronal and synaptic maturation. The purpose of this microiontophoretic study was to explore GABAergic and cholinergic central mechanisms in adult rats exposed to alcohol in the third phase of prenatal life (ADM), when their mothers were subjected to alcohol physical dependence induction (9.6 glkglday). Responses to acetylcholine and GABA were recorded in frontal and somatosensory cortical neurons. Adult rats, whose mothers had been administered placebo with identical procedures, were used as a control (C) Cholinergic responses were significantly decreased and GABAergic responses increased in ADM animals with respect to controls. After a single i.p. alcohol injection (1.6 g/kg) spontaneous firing was depressed in ADM animals to a lesser extent than in C rats. Cholinergic excitations were reduced in C group and potentiated with reversal of atropine antagonism in ADM animals. GABAergic inhibitions were slightly increased and bicuculline antagonism was blocked in C rats, while ADM animals showed decreased responses to GABA. The present results support the hyperactivity of GABAergic system and the hypoactivity of cholinergic system reported in previous studies on prenatally and postnatally alcohol-exposed animals. Microiontophoretic results following ethanol injection led to the hypothesis that a rapid tolerance/dependence may develop in the offspring of alcohol-dependent rats.

Original languageEnglish
Pages (from-to)611-619
Number of pages9
JournalAlcohol and Alcoholism
Volume29
Issue number5
Publication statusPublished - Sep 1994

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ASJC Scopus subject areas

  • Toxicology
  • Psychiatry and Mental health
  • Medicine (miscellaneous)

Cite this

Janiri, L., Gobbi, G., Persico, A. M., Santarelli, M., Minciacchi, D., & Tempesta, E. (1994). Alterations of neocortical neuronal responses to acetylcholine and gaba in rats born to alcohol-dependent mothers. Alcohol and Alcoholism, 29(5), 611-619.