Alterations of RNA metabolism by proteomic analysis of breast cancer cells exposed to marycin: A new optically active porphyrin

Elena Taverna, Maida De Bortoli, Elisa Maffioli, Cristina Corno, Emilio Ciusani, Silvio Trivulzio, Arnaldo Pinelli, Gabriella Tedeschi, Paola Perego, Italia Bongarzone

Research output: Contribution to journalArticle

Abstract

Objective: Marycin is a porphyrin-type compound synthetically modified to spontaneously release fluorescence. This study is aimed at understanding possible mechanisms that could account for the antiproliferative effects observed in marycin. A proteomic approach was used to identify molecular effects. The proteome of proliferating MDA-MB-231 breast cancer cells was compared with that of marycin-treated cells. Methods: Label-free proteomic analysis by liquid chromatography coupled with tandem mass spec-trometry (LC-MS/MS) was used to reveal changes in protein expression and fluorescence microscopy and flow cytometry were used to detect subcellular organelle dysfunctions. Results: The bioinformatic analysis indicated an enhancement of the expression of proteins remodeling RNA splicing and more in general, of RNA metabolism. Marycin did not localize into the mitochondria and did not produce a dramatic increase of ROS levels in MDA-MB-231 cells. Marycin stained organelles probably peroxisomes. Conclusions: The results could support the possibility that the peroxisomes are involved in cell response to marycin.

Original languageEnglish
Pages (from-to)147-159
Number of pages13
JournalCurrent Molecular Pharmacology
Volume12
Issue number2
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Anti-proliferative effect
  • LC-MS/MS
  • Marycin
  • MDA-MB-231 breast cancer cells
  • Peroxisomes
  • Porphyrin
  • RNA metabolism
  • RNA splicing

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

Fingerprint Dive into the research topics of 'Alterations of RNA metabolism by proteomic analysis of breast cancer cells exposed to marycin: A new optically active porphyrin'. Together they form a unique fingerprint.

  • Cite this