Alterations of surface morphology caused by the metabolism of menadione in mammalian cells are associated with the oxidation of critical sulfhydryl groups in cytoskeletal proteins

F. Mirabelli, A. Salis, M. Perotti, F. Taddei, G. Bellomo, S. Orrenius

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Abstract

Incubation of freshly-isolated (rat hepatocytes) or cultured (HeLa, GH3, and McCoy) mammalian cells with menadione (2-methyl-1,4-naphthoquinone) resulted in the appearance of numerous cell surface protrusions. The perturbation of surface structure was associated with an increase in the amount of cytoskeletal protein and the oxidation of sulfhydryl groups in actin, leading to the formation of high-molecular weight aggregates sensitive to treatment with thiol reductants. Our findings indicate that the oxidation of thiol groups in cytoskeletal proteins may be responsible for menadione-induced cell surface abnormalities in mammalian cells.

Original languageEnglish
Pages (from-to)3423-3427
Number of pages5
JournalBiochemical Pharmacology
Volume37
Issue number18
DOIs
Publication statusPublished - Sep 15 1988

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Vitamin K 3
Cytoskeletal Proteins
Metabolism
Surface morphology
Cells
Sulfhydryl Compounds
Oxidation
Cell Surface Extensions
Reducing Agents
Surface structure
Rats
Actins
Hepatocytes
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Pharmacology

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Alterations of surface morphology caused by the metabolism of menadione in mammalian cells are associated with the oxidation of critical sulfhydryl groups in cytoskeletal proteins. / Mirabelli, F.; Salis, A.; Perotti, M.; Taddei, F.; Bellomo, G.; Orrenius, S.

In: Biochemical Pharmacology, Vol. 37, No. 18, 15.09.1988, p. 3423-3427.

Research output: Contribution to journalArticle

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