Alterations of the Plasma Peptidome Profiling in Colorectal Cancer Progression

Chiara Bedin, Sara Crotti, Eugenio Ragazzi, Salvatore Pucciarelli, Lisa Agatea, Ennio Tasciotti, Mauro Ferrari, Pietro Traldi, Flavio Rizzolio, Antonio Giordano, Donato Nitti, Marco Agostini

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Early detection of colorectal cancer (CRC) remains a challenge. It has been highlighted that the pathological alterations within an organ and tissues might be reflected in serum or plasma proteomic/peptidic patterns. The aim of the study was to follow the changes in the plasma peptides associated to colorectal cancer progression by mass spectrometry. This study included 27 adenoma, 67 CRC (n=33 I-II stage and n=34 III-IV stage), 23 liver metastasis from CRC patients and 34 subjects disease-free as controls. For plasma peptides analysis, samples purification was performed on the Nanoporous Silica Chips technology followed by matrix-assisted laser desorption/ionisation-time of flight analysis. Since the high complexity of the obtained dataset, multivariate statistical analysis, and discriminant pattern recognition were performed for study groups classification. Forty-four of 88 ionic species were successfully identified as fragments of peptides and proteins physiologically circulating in the blood and belonging to immune and coagulation systems and inflammatory mediators. Many peptides clustered into sets of overlapping sequences with ladder-like truncation clearly associated to proteolytic processes of both endo- and exoproteases activity. Comparing to controls, a different median ion intensity of the group-type fragments distribution was observed. Moreover, the degradation pattern obtained by proteolytic cleavage was different into study groups. This pattern was specific and characteristic of each group: controls, colon tumour disease (including adenoma and CRC), and liver metastasis, revealing a role as biomarker in early diagnosis and prognosis. Our findings highlighted peculiar changes in protease activity characteristic of CRC progression from pre-cancer lesion to metastatic disease.

Original languageEnglish
JournalJournal of Cellular Physiology
DOIs
Publication statusAccepted/In press - 2015
Externally publishedYes

Fingerprint

Colorectal Neoplasms
Plasmas
Liver
Peptides
Exopeptidases
Peptide Fragments
Ladders
Biomarkers
Coagulation
Silicon Dioxide
Adenoma
Pattern recognition
Ionization
Purification
Mass spectrometry
Tumors
Desorption
Statistical methods
Blood
Peptide Hydrolases

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Bedin, C., Crotti, S., Ragazzi, E., Pucciarelli, S., Agatea, L., Tasciotti, E., ... Agostini, M. (Accepted/In press). Alterations of the Plasma Peptidome Profiling in Colorectal Cancer Progression. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.25196

Alterations of the Plasma Peptidome Profiling in Colorectal Cancer Progression. / Bedin, Chiara; Crotti, Sara; Ragazzi, Eugenio; Pucciarelli, Salvatore; Agatea, Lisa; Tasciotti, Ennio; Ferrari, Mauro; Traldi, Pietro; Rizzolio, Flavio; Giordano, Antonio; Nitti, Donato; Agostini, Marco.

In: Journal of Cellular Physiology, 2015.

Research output: Contribution to journalArticle

Bedin, C, Crotti, S, Ragazzi, E, Pucciarelli, S, Agatea, L, Tasciotti, E, Ferrari, M, Traldi, P, Rizzolio, F, Giordano, A, Nitti, D & Agostini, M 2015, 'Alterations of the Plasma Peptidome Profiling in Colorectal Cancer Progression', Journal of Cellular Physiology. https://doi.org/10.1002/jcp.25196
Bedin, Chiara ; Crotti, Sara ; Ragazzi, Eugenio ; Pucciarelli, Salvatore ; Agatea, Lisa ; Tasciotti, Ennio ; Ferrari, Mauro ; Traldi, Pietro ; Rizzolio, Flavio ; Giordano, Antonio ; Nitti, Donato ; Agostini, Marco. / Alterations of the Plasma Peptidome Profiling in Colorectal Cancer Progression. In: Journal of Cellular Physiology. 2015.
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