Altered glucose catabolism in the presynaptic and perisynaptic compartments of SOD1G93A mouse spinal cord and motor cortex indicates that mitochondria are the site of bioenergetic imbalance in ALS

Silvia Ravera, Carola Torazza, Tiziana Bonifacino, Francesca Provenzano, Claudia Rebosio, Marco Milanese, Cesare Usai, Isabella Panfoli, Giambattista Bonanno

Research output: Contribution to journalArticle

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Abstract

Amyotrophic lateral sclerosis is an adult-onset neurodegenerative disease that develops because of motor neuron death. Several mechanisms occur supporting neurodegeneration, including mitochondrial dysfunction. Recently, we demonstrated that the synaptosomes from the spinal cord of SOD1G93A mice, an in vitro model of presynapses, displayed impaired mitochondrial metabolism at early pre-symptomatic stages of the disease, whereas perisynaptic astrocyte particles, or gliosomes, were characterized by mild energy impairment only at symptomatic stages. This work aimed to understand whether mitochondrial impairment is a consequence of upstream metabolic damage. We analyzed the critical pathways involved in glucose catabolism at presynaptic and perisynaptic compartments. Spinal cord and motor cortex synaptosomes from SOD1G93A mice displayed high activity of hexokinase and phosphofructokinase, key glycolysis enzymes, and of citrate synthase and malate dehydrogenase, key Krebs cycle enzymes, but did not display high lactate dehydrogenase activity, the key enzyme in lactate fermentation. This enhancement was evident in the spinal cord from the early stages of the disease and in the motor cortex at only symptomatic stages. Conversely, an increase in glycolysis and lactate fermentation activity, but not Krebs cycle activity, was observed in gliosomes from the spinal cord and motor cortex of SOD1G93A mice although only at the symptomatic stages of the disease. The cited enzymatic activities were enhanced in spinal cord and motor cortex homogenates, paralleling the time-course of the effect observed in synaptosomes and gliosomes. The observed metabolic modifications might be considered an attempt to restore altered energetic balance and indicate that mitochondria represent the ultimate site of bioenergetic impairment. (Figure presented.).

Original languageEnglish
Pages (from-to)336-350
Number of pages15
JournalJournal of Neurochemistry
Volume151
Issue number3
DOIs
Publication statusPublished - Nov 1 2019

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Mitochondria
Motor Cortex
Energy Metabolism
Spinal Cord
Synaptosomes
Glucose
Citric Acid Cycle
Glycolysis
Fermentation
Lactic Acid
Enzymes
Neurodegenerative diseases
Asymptomatic Diseases
Citrate (si)-Synthase
Phosphofructokinases
Malate Dehydrogenase
Hexokinase
Critical Pathways
Amyotrophic Lateral Sclerosis
Motor Neurons

Keywords

  • gliosomes
  • glycolysis
  • Krebs cycle
  • motor cortex
  • spinal cord
  • synaptosomes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Altered glucose catabolism in the presynaptic and perisynaptic compartments of SOD1G93A mouse spinal cord and motor cortex indicates that mitochondria are the site of bioenergetic imbalance in ALS. / Ravera, Silvia; Torazza, Carola; Bonifacino, Tiziana; Provenzano, Francesca; Rebosio, Claudia; Milanese, Marco; Usai, Cesare; Panfoli, Isabella; Bonanno, Giambattista.

In: Journal of Neurochemistry, Vol. 151, No. 3, 01.11.2019, p. 336-350.

Research output: Contribution to journalArticle

Ravera, Silvia ; Torazza, Carola ; Bonifacino, Tiziana ; Provenzano, Francesca ; Rebosio, Claudia ; Milanese, Marco ; Usai, Cesare ; Panfoli, Isabella ; Bonanno, Giambattista. / Altered glucose catabolism in the presynaptic and perisynaptic compartments of SOD1G93A mouse spinal cord and motor cortex indicates that mitochondria are the site of bioenergetic imbalance in ALS. In: Journal of Neurochemistry. 2019 ; Vol. 151, No. 3. pp. 336-350.
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AU - Ravera, Silvia

AU - Torazza, Carola

AU - Bonifacino, Tiziana

AU - Provenzano, Francesca

AU - Rebosio, Claudia

AU - Milanese, Marco

AU - Usai, Cesare

AU - Panfoli, Isabella

AU - Bonanno, Giambattista

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AB - Amyotrophic lateral sclerosis is an adult-onset neurodegenerative disease that develops because of motor neuron death. Several mechanisms occur supporting neurodegeneration, including mitochondrial dysfunction. Recently, we demonstrated that the synaptosomes from the spinal cord of SOD1G93A mice, an in vitro model of presynapses, displayed impaired mitochondrial metabolism at early pre-symptomatic stages of the disease, whereas perisynaptic astrocyte particles, or gliosomes, were characterized by mild energy impairment only at symptomatic stages. This work aimed to understand whether mitochondrial impairment is a consequence of upstream metabolic damage. We analyzed the critical pathways involved in glucose catabolism at presynaptic and perisynaptic compartments. Spinal cord and motor cortex synaptosomes from SOD1G93A mice displayed high activity of hexokinase and phosphofructokinase, key glycolysis enzymes, and of citrate synthase and malate dehydrogenase, key Krebs cycle enzymes, but did not display high lactate dehydrogenase activity, the key enzyme in lactate fermentation. This enhancement was evident in the spinal cord from the early stages of the disease and in the motor cortex at only symptomatic stages. Conversely, an increase in glycolysis and lactate fermentation activity, but not Krebs cycle activity, was observed in gliosomes from the spinal cord and motor cortex of SOD1G93A mice although only at the symptomatic stages of the disease. The cited enzymatic activities were enhanced in spinal cord and motor cortex homogenates, paralleling the time-course of the effect observed in synaptosomes and gliosomes. The observed metabolic modifications might be considered an attempt to restore altered energetic balance and indicate that mitochondria represent the ultimate site of bioenergetic impairment. (Figure presented.).

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