Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis

Cristina Cereda; Emanuela Leoni; Pamela Milani; Orietta Pansarasa; Giuliano Mazzini; Stefania Guareschi; Elena Alvisi; Andrea Ghiroldi; Luca Diamanti; Stefano Bernuzzi; Mauro Ceroni; Emanuela Cova

doi:10.1371/journal.pone.0075916

Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis

Cristina Cereda, Emanuela Leoni, Pamela Milani, Orietta Pansarasa, Giuliano Mazzini, Stefania Guareschi, Elena Alvisi, Andrea Ghiroldi, Luca Diamanti, Stefano Bernuzzi, Mauro Ceroni, Emanuela Cova

Research output: Contribution to journal › Article › peer-review

Abstract

Several lines of evidence support the hypothesis of a toxic role played by wild type SOD1 (WT-SOD1) in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). In this study we investigated both distribution and expression profile of WT-SOD1 in leukocytes from 19 SALS patients and 17 healthy individuals. Immunofluorescence experiments by confocal microscopy showed that SOD1 accumulates in the nuclear compartment in a group of SALS subjects. These results were also confirmed by western blot carried out on soluble nuclear and cytoplasmic fractions, with increased nuclear SOD1 level (p

Original language	English
Article number	e75916
Journal	PLoS One
Volume	8
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0075916
Publication status	Published - Oct 14 2013

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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abstract = "Several lines of evidence support the hypothesis of a toxic role played by wild type SOD1 (WT-SOD1) in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). In this study we investigated both distribution and expression profile of WT-SOD1 in leukocytes from 19 SALS patients and 17 healthy individuals. Immunofluorescence experiments by confocal microscopy showed that SOD1 accumulates in the nuclear compartment in a group of SALS subjects. These results were also confirmed by western blot carried out on soluble nuclear and cytoplasmic fractions, with increased nuclear SOD1 level (p",

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AU - Cereda, Cristina

AU - Leoni, Emanuela

AU - Milani, Pamela

AU - Pansarasa, Orietta

AU - Mazzini, Giuliano

AU - Guareschi, Stefania

AU - Alvisi, Elena

AU - Ghiroldi, Andrea

AU - Diamanti, Luca

AU - Bernuzzi, Stefano

AU - Ceroni, Mauro

AU - Cova, Emanuela

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Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis

Abstract

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