Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis

Cristina Cereda, Emanuela Leoni, Pamela Milani, Orietta Pansarasa, Giuliano Mazzini, Stefania Guareschi, Elena Alvisi, Andrea Ghiroldi, Luca Diamanti, Stefano Bernuzzi, Mauro Ceroni, Emanuela Cova

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Abstract

Several lines of evidence support the hypothesis of a toxic role played by wild type SOD1 (WT-SOD1) in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). In this study we investigated both distribution and expression profile of WT-SOD1 in leukocytes from 19 SALS patients and 17 healthy individuals. Immunofluorescence experiments by confocal microscopy showed that SOD1 accumulates in the nuclear compartment in a group of SALS subjects. These results were also confirmed by western blot carried out on soluble nuclear and cytoplasmic fractions, with increased nuclear SOD1 level (p

Original languageEnglish
Article numbere75916
JournalPLoS One
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 14 2013

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Confocal microscopy
Poisons
leukocytes
Leukocytes
Experiments
Confocal Microscopy
fluorescent antibody technique
Fluorescent Antibody Technique
Western blotting
pathogenesis
Western Blotting
Amyotrophic lateral sclerosis 1
amyotrophic lateral sclerosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis. / Cereda, Cristina; Leoni, Emanuela; Milani, Pamela; Pansarasa, Orietta; Mazzini, Giuliano; Guareschi, Stefania; Alvisi, Elena; Ghiroldi, Andrea; Diamanti, Luca; Bernuzzi, Stefano; Ceroni, Mauro; Cova, Emanuela.

In: PLoS One, Vol. 8, No. 10, e75916, 14.10.2013.

Research output: Contribution to journalArticle

Cereda, Cristina ; Leoni, Emanuela ; Milani, Pamela ; Pansarasa, Orietta ; Mazzini, Giuliano ; Guareschi, Stefania ; Alvisi, Elena ; Ghiroldi, Andrea ; Diamanti, Luca ; Bernuzzi, Stefano ; Ceroni, Mauro ; Cova, Emanuela. / Altered Intracellular Localization of SOD1 in Leukocytes from Patients with Sporadic Amyotrophic Lateral Sclerosis. In: PLoS One. 2013 ; Vol. 8, No. 10.
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