Altered neuron excitability and synaptic plasticity in the cerebellar granular layer of juvenile prion protein knock-out mice with impaired motor control

Francesca Prestori, Paola Rossi, Bertrand Bearzatto, Jeanne Lainé, Daniela Necchi, Shyam Diwakar, Serge N. Schiffmann, Herbert Axelrad, Egidio D'Angelo

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Although the role of abnormal prion protein (PrP) conformation in generating infectious brain diseases (transmissible spongiform encephalopathy) has been recognized, the function of PrP in the normal brain remains mostly unknown. In this investigation, we considered the effect of PrP gene knock-out (PrP0/0) on cerebellar neural circuits and in particular on granule cells, which show intense PrP expression during development and selective affinity for PrP. At the third postnatal week, when PrP expression would normally attain mature levels, PrP0/0 mice showed low performance in the accelerating rotarod and runway tests and the functioning of 40% of granule cells was abnormal. Spikes were slow, nonovershooting, and nonrepetitive in relation with a reduction in transient inward and outward membrane currents, and also the EPSPs and EPSCs had slow kinetics. Overall, these alterations closely resembled an immature phenotype. Moreover, in slow-spiking PrP0/0 granule cells, theta-burst stimulation was unable to induce any long-term potentiation. This profound impairment in synaptic excitation and plasticity was associated with a protracted proliferation of granule cells and disappeared at P40-P50 along with the recovery of normal motor behavior (Büeler et al., 1992). These results suggest that PrP plays an important role in granule cell development eventually regulating cerebellar network formation and motor control.

Original languageEnglish
Pages (from-to)7091-7103
Number of pages13
JournalJournal of Neuroscience
Volume28
Issue number28
DOIs
Publication statusPublished - Jul 9 2008

Fingerprint

Neuronal Plasticity
Knockout Mice
Neurons
Rotarod Performance Test
Gene Knockout Techniques
Protein Conformation
Prion Diseases
Long-Term Potentiation
Excitatory Postsynaptic Potentials
Brain Diseases
Prion Proteins
Communicable Diseases
Cell Proliferation
Phenotype
Membranes
Brain

Keywords

  • Action potential
  • Cerebellum
  • Granule cell
  • LTP
  • Motor control
  • Prion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Altered neuron excitability and synaptic plasticity in the cerebellar granular layer of juvenile prion protein knock-out mice with impaired motor control. / Prestori, Francesca; Rossi, Paola; Bearzatto, Bertrand; Lainé, Jeanne; Necchi, Daniela; Diwakar, Shyam; Schiffmann, Serge N.; Axelrad, Herbert; D'Angelo, Egidio.

In: Journal of Neuroscience, Vol. 28, No. 28, 09.07.2008, p. 7091-7103.

Research output: Contribution to journalArticle

Prestori, Francesca ; Rossi, Paola ; Bearzatto, Bertrand ; Lainé, Jeanne ; Necchi, Daniela ; Diwakar, Shyam ; Schiffmann, Serge N. ; Axelrad, Herbert ; D'Angelo, Egidio. / Altered neuron excitability and synaptic plasticity in the cerebellar granular layer of juvenile prion protein knock-out mice with impaired motor control. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 28. pp. 7091-7103.
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