Abstract
Through the genotype/phenotype cosegregation analysis of an F2 intercross, from the crossbreeding of stroke-prone spontaneously hypertensive rats (SHRSP) and stroke-resistant spontaneously hypertensive rats (SHR), we previously identified a quantitative trait locus for stroke on rat chromosome 5 (STR2) that colocalized with the genes encoding atrial and brain natriuretic peptides (ANP and BNP) and conferred a stroke-delaying effect. To further characterize ANP and BNP as candidates for stroke, we performed additional studies. Comparative sequence analysis revealed point mutations in both the coding and regulatory regions of ANP, whereas no interstrain differences were found for BNP. In in vitro studies in COS-7 and AtT-20 cells that were performed to test the relevance of a G→A substitution at position 1125, a Gly→Ser transposition in the SHRSP pro-ANP peptide resulted in different posttranslational processing of the SHRSP ANP gene product that was also associated with higher cGMP production (P
| Original language | English |
|---|---|
| Pages (from-to) | 900-905 |
| Number of pages | 6 |
| Journal | Circulation Research |
| Volume | 85 |
| Issue number | 10 |
| Publication status | Published - Nov 12 1999 |
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Keywords
- Atrial natriuretic peptide
- Genetics
- Natriuretic peptide
- Stroke
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Altered structure, regulation, and function of the gene encoding the atrial natriuretic peptide in the stroke-prone spontaneously hypertensive rat. / Rubattu, Speranza; Lee-Kirsch, Min Ae; DePaolis, Paola; Giliberti, Rosangela; Gigante, Bruna; Lombardi, Alessia; Volpe, Massimo; Lindpaintner, Klaus.
In: Circulation Research, Vol. 85, No. 10, 12.11.1999, p. 900-905.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Altered structure, regulation, and function of the gene encoding the atrial natriuretic peptide in the stroke-prone spontaneously hypertensive rat
AU - Rubattu, Speranza
AU - Lee-Kirsch, Min Ae
AU - DePaolis, Paola
AU - Giliberti, Rosangela
AU - Gigante, Bruna
AU - Lombardi, Alessia
AU - Volpe, Massimo
AU - Lindpaintner, Klaus
PY - 1999/11/12
Y1 - 1999/11/12
N2 - Through the genotype/phenotype cosegregation analysis of an F2 intercross, from the crossbreeding of stroke-prone spontaneously hypertensive rats (SHRSP) and stroke-resistant spontaneously hypertensive rats (SHR), we previously identified a quantitative trait locus for stroke on rat chromosome 5 (STR2) that colocalized with the genes encoding atrial and brain natriuretic peptides (ANP and BNP) and conferred a stroke-delaying effect. To further characterize ANP and BNP as candidates for stroke, we performed additional studies. Comparative sequence analysis revealed point mutations in both the coding and regulatory regions of ANP, whereas no interstrain differences were found for BNP. In in vitro studies in COS-7 and AtT-20 cells that were performed to test the relevance of a G→A substitution at position 1125, a Gly→Ser transposition in the SHRSP pro-ANP peptide resulted in different posttranslational processing of the SHRSP ANP gene product that was also associated with higher cGMP production (P
AB - Through the genotype/phenotype cosegregation analysis of an F2 intercross, from the crossbreeding of stroke-prone spontaneously hypertensive rats (SHRSP) and stroke-resistant spontaneously hypertensive rats (SHR), we previously identified a quantitative trait locus for stroke on rat chromosome 5 (STR2) that colocalized with the genes encoding atrial and brain natriuretic peptides (ANP and BNP) and conferred a stroke-delaying effect. To further characterize ANP and BNP as candidates for stroke, we performed additional studies. Comparative sequence analysis revealed point mutations in both the coding and regulatory regions of ANP, whereas no interstrain differences were found for BNP. In in vitro studies in COS-7 and AtT-20 cells that were performed to test the relevance of a G→A substitution at position 1125, a Gly→Ser transposition in the SHRSP pro-ANP peptide resulted in different posttranslational processing of the SHRSP ANP gene product that was also associated with higher cGMP production (P
KW - Atrial natriuretic peptide
KW - Genetics
KW - Natriuretic peptide
KW - Stroke
UR - http://www.scopus.com/inward/record.url?scp=0032719444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032719444&partnerID=8YFLogxK
M3 - Article
C2 - 10559136
AN - SCOPUS:0032719444
VL - 85
SP - 900
EP - 905
JO - Circulation Research
JF - Circulation Research
SN - 0009-7330
IS - 10
ER -