Alternative source of stem cells derived from human periodontal ligament: A new treatment for experimental autoimmune encephalomyelitis

Oriana Trubiani, Sabrina Giacoppo, Patrizia Ballerini, Francesca Diomede, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon

Research output: Contribution to journalArticle

Abstract

Background: Multiple sclerosis is a demyelinating disease mostly of autoimmune origin that affects and damages the central nervous system, leading to a disabling condition. The aim of the present study was to investigate whether administration of mesenchymal stem cells from human periodontal ligament (hPDLSCs) could ameliorate multiple sclerosis progression by exerting neuroprotective effects in an experimental model of autoimmune encephalomyelitis (EAE). Methods: EAE was induced by immunization with myelin oligodendroglial glycoprotein peptide (MOG)35-55 in C57BL/6 mice. After immunization, mice were observed every 48 hours for signs of EAE and weight loss. At the onset of disease, approximately 14 days after immunization, EAE mice were subjected to a single intravenous injection of hPDLSCs (106 cells/150 μl) into the tail vein. At the point of animal sacrifice on day 56 after EAE induction, spinal cord and brain tissues were collected in order to perform histological evaluation, immunohistochemistry and western blotting analysis. Results: Achieved results reveal that treatment with hPDLSCs may exert neuroprotective effects against EAE, diminishing both clinical signs and histological score typical of the disease (lymphocytic infiltration and demyelination) probably through the production of neurotrophic factors (results focused on brain-derived neurotrophic factor and nerve growth factor expression). Furthermore, administration of hPDLSCs modulates expression of inflammatory key markers (tumor necrosis factor-α, interleukin (IL)-1β, IL-10, glial fibrillary acidic protein, Nrf2 and Foxp3), the release of CD4 and CD8α T cells, and the triggering of apoptotic death pathway (data shown for cleaved caspase 3, p53 and p21). Conclusions: In light of the achieved results, transplantation of hPDLSCs may represent a putative novel and helpful tool for multiple sclerosis treatment. These cells could have considerable implication for future therapies for multiple sclerosis and this study may represent the starting point for further investigations.

Original languageEnglish
Article number1
JournalStem Cell Research and Therapy
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 4 2016

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Immunization
Encephalomyelitis
Periodontal Ligament
Autoimmune Experimental Encephalomyelitis
Ligaments
Stem cells
Stem Cells
Neuroprotective Agents
Multiple Sclerosis
T-cells
Glial Fibrillary Acidic Protein
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Neurology
Demyelinating Diseases
Nerve Growth Factor
Interleukin-1
Infiltration
Caspase 3
Interleukin-10

Keywords

  • Apoptosis
  • Multiple sclerosis
  • Neurotrophic factors
  • Stem cells derived from human periodontal ligament

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)

Cite this

Alternative source of stem cells derived from human periodontal ligament : A new treatment for experimental autoimmune encephalomyelitis. / Trubiani, Oriana; Giacoppo, Sabrina; Ballerini, Patrizia; Diomede, Francesca; Piattelli, Adriano; Bramanti, Placido; Mazzon, Emanuela.

In: Stem Cell Research and Therapy, Vol. 7, No. 1, 1, 04.01.2016.

Research output: Contribution to journalArticle

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