Alternative splicing in adhesion- and motility-related genes in breast cancer

Rosanna Aversa, Anna Sorrentino, Roberta Esposito, Maria Rosaria Ambrosio, Angela Amato, Alberto Zambelli, Alfredo Ciccodicola, Luciana D’Apice, Valerio Costa

Research output: Contribution to journalArticle

Abstract

Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression.

Original languageEnglish
Article number121
JournalInternational Journal of Molecular Sciences
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 16 2016

Fingerprint

splicing
locomotion
Alternative Splicing
Semaphorins
breast
genes
adhesion
Adhesion
Genes
cancer
Breast Neoplasms
Tumors
Molecules
Cells
Protein Isoforms
tumors
Neoplasms
Breast
Gene encoding
Biopsy

Keywords

  • Alternative splicing
  • Breast cancer
  • Cell adhesion and motility
  • RNA-Sequencing

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

Aversa, R., Sorrentino, A., Esposito, R., Ambrosio, M. R., Amato, A., Zambelli, A., ... Costa, V. (2016). Alternative splicing in adhesion- and motility-related genes in breast cancer. International Journal of Molecular Sciences, 17(1), [121]. https://doi.org/10.3390/ijms17010121

Alternative splicing in adhesion- and motility-related genes in breast cancer. / Aversa, Rosanna; Sorrentino, Anna; Esposito, Roberta; Ambrosio, Maria Rosaria; Amato, Angela; Zambelli, Alberto; Ciccodicola, Alfredo; D’Apice, Luciana; Costa, Valerio.

In: International Journal of Molecular Sciences, Vol. 17, No. 1, 121, 16.01.2016.

Research output: Contribution to journalArticle

Aversa, R, Sorrentino, A, Esposito, R, Ambrosio, MR, Amato, A, Zambelli, A, Ciccodicola, A, D’Apice, L & Costa, V 2016, 'Alternative splicing in adhesion- and motility-related genes in breast cancer', International Journal of Molecular Sciences, vol. 17, no. 1, 121. https://doi.org/10.3390/ijms17010121
Aversa R, Sorrentino A, Esposito R, Ambrosio MR, Amato A, Zambelli A et al. Alternative splicing in adhesion- and motility-related genes in breast cancer. International Journal of Molecular Sciences. 2016 Jan 16;17(1). 121. https://doi.org/10.3390/ijms17010121
Aversa, Rosanna ; Sorrentino, Anna ; Esposito, Roberta ; Ambrosio, Maria Rosaria ; Amato, Angela ; Zambelli, Alberto ; Ciccodicola, Alfredo ; D’Apice, Luciana ; Costa, Valerio. / Alternative splicing in adhesion- and motility-related genes in breast cancer. In: International Journal of Molecular Sciences. 2016 ; Vol. 17, No. 1.
@article{4d2253957a8341fcb58a867f89c30693,
title = "Alternative splicing in adhesion- and motility-related genes in breast cancer",
abstract = "Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression.",
keywords = "Alternative splicing, Breast cancer, Cell adhesion and motility, RNA-Sequencing",
author = "Rosanna Aversa and Anna Sorrentino and Roberta Esposito and Ambrosio, {Maria Rosaria} and Angela Amato and Alberto Zambelli and Alfredo Ciccodicola and Luciana D’Apice and Valerio Costa",
year = "2016",
month = "1",
day = "16",
doi = "10.3390/ijms17010121",
language = "English",
volume = "17",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "1",

}

TY - JOUR

T1 - Alternative splicing in adhesion- and motility-related genes in breast cancer

AU - Aversa, Rosanna

AU - Sorrentino, Anna

AU - Esposito, Roberta

AU - Ambrosio, Maria Rosaria

AU - Amato, Angela

AU - Zambelli, Alberto

AU - Ciccodicola, Alfredo

AU - D’Apice, Luciana

AU - Costa, Valerio

PY - 2016/1/16

Y1 - 2016/1/16

N2 - Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression.

AB - Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression.

KW - Alternative splicing

KW - Breast cancer

KW - Cell adhesion and motility

KW - RNA-Sequencing

UR - http://www.scopus.com/inward/record.url?scp=84954327354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954327354&partnerID=8YFLogxK

U2 - 10.3390/ijms17010121

DO - 10.3390/ijms17010121

M3 - Article

AN - SCOPUS:84954327354

VL - 17

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 1

M1 - 121

ER -