TY - JOUR
T1 - Alternative splicing of hTERT
T2 - a further mechanism for the control of active hTERT in acute myeloid leukemia
AU - Calvello, Celeste
AU - Rocca, Barbara
AU - Klersy, Catherine
AU - Zappatore, Rita
AU - Giardini, Ilaria
AU - Dambruoso, Irene
AU - Pasi, Francesca
AU - Caresana, Marilena
AU - Zappasodi, Patrizia
AU - Nano, Rosanna
AU - Boni, Marina
AU - Bernasconi, Paolo
PY - 2018/3/4
Y1 - 2018/3/4
N2 - hTERT component is the key regulator of telomerase. Alternatively spliced variants of hTERT generate different telomerase activity. The goal of the study was to determine the role of different hTERT isoforms in the regulation of telomerase expression in AML patients. Among the 97 studied patients, 45 had a complex karyotype and 52 a normal karyotype. hTERT isoforms expression was determined in bone marrow samples by q-RT-PCR, using SYBR Green I. hTERT expression was lower in AML patients than controls (median 2.5 vs. 10.1, p =.003), though no difference was observed between the complex and normal karyotype (median 3.2 vs. 2.3, p =.37). High trans-dominant negative isoform expression increased the response rate by two. High expression of inactive product (–α–β) was shown to increase the risk of relapse by about three times. In conclusion, our data suggest an intriguing link between the control of hTERT isoforms expression and AML outcome.
AB - hTERT component is the key regulator of telomerase. Alternatively spliced variants of hTERT generate different telomerase activity. The goal of the study was to determine the role of different hTERT isoforms in the regulation of telomerase expression in AML patients. Among the 97 studied patients, 45 had a complex karyotype and 52 a normal karyotype. hTERT isoforms expression was determined in bone marrow samples by q-RT-PCR, using SYBR Green I. hTERT expression was lower in AML patients than controls (median 2.5 vs. 10.1, p =.003), though no difference was observed between the complex and normal karyotype (median 3.2 vs. 2.3, p =.37). High trans-dominant negative isoform expression increased the response rate by two. High expression of inactive product (–α–β) was shown to increase the risk of relapse by about three times. In conclusion, our data suggest an intriguing link between the control of hTERT isoforms expression and AML outcome.
KW - acute myeloid leukemia
KW - hTERT
KW - telomerase
UR - http://www.scopus.com/inward/record.url?scp=85021912110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021912110&partnerID=8YFLogxK
U2 - 10.1080/10428194.2017.1346252
DO - 10.1080/10428194.2017.1346252
M3 - Article
AN - SCOPUS:85021912110
VL - 59
SP - 702
EP - 709
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
SN - 1042-8194
IS - 3
ER -