Alternative splicing of human G6PD messenger RNA in K562 cells but not in cultured erythroblasts

M. D. Cappellini, D. Tavazzi, F. Martinez Di Montemuros, M. Sampietro, A. Gaviraghi, D. Carandini, G. Fiorelli

Research output: Contribution to journalArticle

Abstract

The biochemical properties of glucose-6-phosphate dehydrogenase (G6PD) vary in different tissues, and different protein isoforms of the enzyme have been described. Alternative splicing of G6PD intron VII has been detected in transformed lymphoblasts, granulocytes and spermatocytes; the function of this mRNA species is still unknown. We developed a PCR for detecting alternatively spliced G6PD mRNA in K562 and in erythroblasts at different stage of maturation obtained from human peripheral BFU-E in order to evaluate a possible physiological role during erythroid maturation. Trace events of alternative splicing of G6PD intron VII sequences were observed in K562 cells but not in BFU-E-derived erythroid precursors; we consider this phenomenon a non-functional activity in the cells analysed.

Original languageEnglish
Pages (from-to)188-191
Number of pages4
JournalEuropean Journal of Clinical Investigation
Volume23
Issue number3
Publication statusPublished - 1993

Keywords

  • alternative splicing
  • G6PD mRNA erythroblasts

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Cappellini, M. D., Tavazzi, D., Martinez Di Montemuros, F., Sampietro, M., Gaviraghi, A., Carandini, D., & Fiorelli, G. (1993). Alternative splicing of human G6PD messenger RNA in K562 cells but not in cultured erythroblasts. European Journal of Clinical Investigation, 23(3), 188-191.