Alu and LINE-1 methylation and lung function in the normative ageing study

Nancy E. Lange, Joanne Sordillo, Letizia Tarantini, Valentina Bollati, David Sparrow, Pantel Vokonas, Antonella Zanobetti, Joel Schwartz, Andrea Baccarelli, Augusto A. Litonjua, Dawn L. DeMeo

Research output: Contribution to journalArticle

Abstract

Objectives: To investigate the association between methylation of transposable elements Alu and long-interspersed nuclear elements (LINE-1) and lung function. Design: Cohort study. Setting: Outpatient Veterans Administration facilities in greater Boston, Massachusetts, USA. Participants: Individuals from the Veterans Administration Normative Aging Study, a longitudinal study of aging in men, evaluated between 1999 and 2007. The majority (97%) were white. Primary and secondary outcome measures: Primary predictor was methylation, assessed using PCR-pyrosequencing after bisulphite treatment. Primary outcome was lung function as assessed by spirometry, performed according to American Thoracic Society/European Respiratory Society guidelines at the same visit as the blood draws. Results: In multivariable models adjusted for age, height, body mass index (BMI), pack-years of smoking, current smoking and race, Alu hypomethylation was associated with lower forced expiratory volume in 1 s (FEV1) (β=28 ml per 1% change in Alu methylation, p=0.017) and showed a trend towards association with a lower forced vital capacity (FVC) (β=27 ml, p=0.06) and lower FEV1/FVC (β=0.3%, p=0.058). In multivariable models adjusted for age, height, BMI, pack-years of smoking, current smoking, per cent lymphocytes, race and baseline lung function, LINE-1 hypomethylation was associated with more rapid decline of FEV1 (β=6.9 ml/year per 1% change in LINE-1 methylation, p=0.005) and of FVC (β=9.6 ml/year, p=0.002). Conclusions: In multiple regression analysis, Alu hypomethylation was associated with lower lung function, and LINE-1 hypomethylation was associated with more rapid lung function decline in a cohort of older and primarily white men from North America. Future studies should aim to replicate these findings and determine if Alu or LINE-1 hypomethylation may be due to specific and modifiable environmental exposures.

Original languageEnglish
Article numbere001231
JournalBMJ Open
Volume2
Issue number5
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Alu and LINE-1 methylation and lung function in the normative ageing study'. Together they form a unique fingerprint.

  • Cite this

    Lange, N. E., Sordillo, J., Tarantini, L., Bollati, V., Sparrow, D., Vokonas, P., Zanobetti, A., Schwartz, J., Baccarelli, A., Litonjua, A. A., & DeMeo, D. L. (2012). Alu and LINE-1 methylation and lung function in the normative ageing study. BMJ Open, 2(5), [e001231]. https://doi.org/10.1136/bmjopen-2012-001231