Alzheimer patients: Preamyloid deposits are immunoreactive with antibodies to extracellular domains of the amyloid precursor protein

Fabrizio Tagliavini, Giorgio Giaccone, Laura Verga, Jorge Ghiso, Blas Frangione, Orso Bugiani

Research output: Contribution to journalArticlepeer-review

Abstract

In patients with Alzheimer's disease, in patients with Down's syndrome and in aged non-demented individuals, anti-β-protein antibodies label not only the fibrillary amyloid, but also preamyloid deposits. The latter are made up of amorphous material lacking the tinctorial, optical and ultrastructural properties of amyloid fibrils. To investigate the antigenic profile of preamyloid deposits, we have carried out an immunohistochemical study on specimens of cerebral cortex from 4 Alzheimer patients and two non-demented individuals, using antibodies to the β-protein (anti-SP28), the C-terminal region of the amyloid precursor protein (APP) (anti-SP20) and an APP extracellular epitope between residues 50 and 100 (anti-preA4). Anti-preA4 and anti-SP28 immunoreactivity was found to be present in preamyloid deposits, whereas anti-SP20 immunoreactivity was not. These findings suggest that an extracellular portion of APP, close to the N-terminus of the molecule, participates with β-protein in the composition of premyloid deposits.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalNeuroscience Letters
Volume128
Issue number1
DOIs
Publication statusPublished - Jul 8 1991

Keywords

  • Alzheimer's disease
  • Amyloid
  • Amyloid precursor protein
  • Down's syndrome
  • Preamyloid deposit

ASJC Scopus subject areas

  • Neuroscience(all)

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