Amifostine (WR2721) restores transcriptional activity of specific p53 mutant proteins in a yeast functional assay

Daniela Maurici, Paola Monti, Paola Campomenosi, Sophie North, Thierry Frebourg, Gilberto Fronza, Pierre Hainaut

Research output: Contribution to journalArticlepeer-review

Abstract

Many p53 mutants found in human cancer have an altered ability to bind DNA and transactivate gene expression. Re-expression of functional p53 in cells in which the endogenous TP53 gene is inactivated has been demonstrated to restore a non-tumorigenic phenotype. Pharmacological modulation of p53 mutant conformation may therefore represent a mechanism to reactivate p53 function and consequently improve response to radio- and chemotherapy. We have recently reported that the radio- and chemoprotector Amifostine (WR2721, Ethyol®) activates wild-type p53 in cultured mammalian cells. In the present study, we have used a yeast functional assay to investigate the effect of WR2721 on the transcriptional activity of p53. WR2721 restored this activity in a temperature-sensitive mutant V272M (valine to methionine at codon 272) expressed at the non-permissive temperature and it also partially restored the transcriptional activity of several other conformationally flexible p53 mutants. The results indicate that the yeast functional assay may be used to identify compounds that modulate p53 activity, with potential therapeutic implications.

Original languageEnglish
Pages (from-to)3533-3540
Number of pages8
JournalOncogene
Volume20
Issue number27
DOIs
Publication statusPublished - Jun 14 2001

Keywords

  • Amifostine
  • p53
  • Yeast functional assay

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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