Amine oxidase distribution in different organs of lentil seedlings

M. Maccarrone, M. R. Bladergroen, A. Rossi, A. F. Agro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lentil (Lens culinaris L.) seedlings were grown in the dark and were cut into different organs, i.e. roots, cotyledons, epicotyls and leaves. Amine oxidase activity in each organ was determined and the samples showing the highest activities were subjected to electrophoretic and immunochemical analysis. Amine oxidases from the different lentil organs showed the same molecular mass (67 kDa/subunit), but different isoelectric points : 6.25 (amine oxidases from epicotyls and leaves) and 6.50 (amine oxidases from cotyledons and roots). Moreover, the amine oxidases responded differently to water flooding/deficit stresses. These results suggest that different isoenzymes are present in lentil organs. Finally, in lentil protoplasts amine oxidase was localized in the cell wall and was not present in the cytosol.

Original languageEnglish
Pages (from-to)473-478
Number of pages6
JournalPlant Physiology and Biochemistry
Volume34
Issue number4
Publication statusPublished - 1996

Fingerprint

Lens Plant
lentils
amines
Seedlings
Amines
Oxidoreductases
seedlings
epicotyls
Cotyledon
cotyledons
Lens culinaris
Protoplasts
Isoelectric Point
Molecular mass
isoelectric point
cytosol
Cytosol
Cell Wall
protoplasts
Isoenzymes

Keywords

  • Amine oxidase
  • isoenzymes
  • Lens culinaris
  • protoplast
  • water stress

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Amine oxidase distribution in different organs of lentil seedlings. / Maccarrone, M.; Bladergroen, M. R.; Rossi, A.; Agro, A. F.

In: Plant Physiology and Biochemistry, Vol. 34, No. 4, 1996, p. 473-478.

Research output: Contribution to journalArticle

Maccarrone, M, Bladergroen, MR, Rossi, A & Agro, AF 1996, 'Amine oxidase distribution in different organs of lentil seedlings', Plant Physiology and Biochemistry, vol. 34, no. 4, pp. 473-478.
Maccarrone, M. ; Bladergroen, M. R. ; Rossi, A. ; Agro, A. F. / Amine oxidase distribution in different organs of lentil seedlings. In: Plant Physiology and Biochemistry. 1996 ; Vol. 34, No. 4. pp. 473-478.
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