Amino acid- and lipid-induced insulin resistance in rat heart: Molecular mechanisms

Ileana Terruzzi, Sonia Allibardi, Paola Bendinelli, Paola Maroni, Roberta Piccoletti, Flavio Vesco, Michele Samaja, Livio Luzi

Research output: Contribution to journalArticlepeer-review

Abstract

Lipids compete with glucose for utilization by the myocardium. Amino acids are an important energetic substrate in the heart but it is unknown whether they reduce glucose disposal. The molecular mechanisms by which lipids and amino acids impair insulin-mediated glucose disposal in the myocardium are unknown. We evaluated the effect of lipids and amino acids on the insulin stimulated glucose uptake in the isolated rat heart and explored the involved target proteins. The hearts were perfused with 16 mM glucose alone or with 6% lipid or 10% amino acid solutions at the rate of 15 ml/min. After 1 h of perfusion (basal period), insulin (240 nmol/l) was added and maintained for an additional hour. Both lipids and amino acids blocked the insulin effect on glucose uptake (P

Original languageEnglish
Pages (from-to)135-145
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume190
Issue number1-2
DOIs
Publication statusPublished - Apr 25 2002

Keywords

  • Amino acids
  • Heart
  • Insulin resistance
  • Kinases
  • Lipids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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